Photosynthesis Methods and Protocols
Photosynthesis Methods and Protocols
edited by Sarah Covshoff.
- 1st edition 2018
- 1 recurso en línea (XI, 355 páginas) 70 ilustraciones, 47 ilustraciones a color
- Methods in Molecular Biology 1770 1940-6029 .
Survey of Tools for Measuring In Vivo Photosynthesis -- Photosynthetic Gas Exchange in Land Plants at the Leaf Level -- Design and Use of a Digitally-Controlled Device for Accurate, Multiplexed Gas Exchange Measurements of the Complete Foliar Parts of Plants -- Measuring Canopy Gas Exchange Using CAnopy Photosynthesis and Transpiration Systems (CAPTS) -- Light-Response Curves in Land Plants -- Chlorophyll Fluorescence on the Fast Timescale -- Sub-Saturating Multiphase Flash Irradiances to Estimate Maximum Fluorescence Yield -- Chlorophyll Fluorescence Imaging -- Measurement of O2 Uptake and Evolution in Leaves In Vivo Using Stable Isotopes and Membrane Inlet Mass Spectrometry -- Using Carbon Stable Isotopes to Study C3 and C4 Photosynthesis: Models and Calculations.- Liquid-Phase Measurements of Photosynthetic Oxygen Evolution.- Quantification of Photosynthetic Enzymes in Leaf Extracts by Immunoblotting -- Extraction of Rubisco to Determine Catalytic Constants -- Spectrophotometric Determination of Rubisco Activity and Activation State in Leaf Extracts -- Creating Leaf Cell Suspensions for Characterization of Mesophyll and Bundle Sheath Cellular Features.- Determining the Subcellular Localization of Fluorescently-Tagged Proteins Using Protoplasts Extracted from Transiently-Transformed Nicotiana benthamiana Leaves.- 3D Clearing and Molecular Labeling in Plant Tissues -- Evaluation of Lipids for the Study of Photosynthetic Membranes -- "Click" Methodology for the Functionalization of Water Oxidation Catalyst Iridium Oxide Nanoparticles with Hydrophobic Dyes for Artificial Photosynthetic Constructs -- Biophotovoltaics: Design and Study of Bio-Electrochemical Systems for Biotechnological Applications and Metabolic Investigation.
This user-friendly book provides a range of classical and modern techniques for the study of photosynthesis in a manner accessible to a broad spectrum of researchers. Broken into four sections, it explores the measurement of physiological photosynthetic parameters, quantifying photosynthetic enzyme abundance and catalytic activity, visualizing cellular and sub-cellular phenotypes, and photosynthesis-inspired energy generation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Photosynthesis: Methods and Protocols aims to aid scientists working toward exciting developments in the understanding and application of photosynthesis.
9781493977864
10.1007/978-1-4939-7786-4 doi
Survey of Tools for Measuring In Vivo Photosynthesis -- Photosynthetic Gas Exchange in Land Plants at the Leaf Level -- Design and Use of a Digitally-Controlled Device for Accurate, Multiplexed Gas Exchange Measurements of the Complete Foliar Parts of Plants -- Measuring Canopy Gas Exchange Using CAnopy Photosynthesis and Transpiration Systems (CAPTS) -- Light-Response Curves in Land Plants -- Chlorophyll Fluorescence on the Fast Timescale -- Sub-Saturating Multiphase Flash Irradiances to Estimate Maximum Fluorescence Yield -- Chlorophyll Fluorescence Imaging -- Measurement of O2 Uptake and Evolution in Leaves In Vivo Using Stable Isotopes and Membrane Inlet Mass Spectrometry -- Using Carbon Stable Isotopes to Study C3 and C4 Photosynthesis: Models and Calculations.- Liquid-Phase Measurements of Photosynthetic Oxygen Evolution.- Quantification of Photosynthetic Enzymes in Leaf Extracts by Immunoblotting -- Extraction of Rubisco to Determine Catalytic Constants -- Spectrophotometric Determination of Rubisco Activity and Activation State in Leaf Extracts -- Creating Leaf Cell Suspensions for Characterization of Mesophyll and Bundle Sheath Cellular Features.- Determining the Subcellular Localization of Fluorescently-Tagged Proteins Using Protoplasts Extracted from Transiently-Transformed Nicotiana benthamiana Leaves.- 3D Clearing and Molecular Labeling in Plant Tissues -- Evaluation of Lipids for the Study of Photosynthetic Membranes -- "Click" Methodology for the Functionalization of Water Oxidation Catalyst Iridium Oxide Nanoparticles with Hydrophobic Dyes for Artificial Photosynthetic Constructs -- Biophotovoltaics: Design and Study of Bio-Electrochemical Systems for Biotechnological Applications and Metabolic Investigation.
This user-friendly book provides a range of classical and modern techniques for the study of photosynthesis in a manner accessible to a broad spectrum of researchers. Broken into four sections, it explores the measurement of physiological photosynthetic parameters, quantifying photosynthetic enzyme abundance and catalytic activity, visualizing cellular and sub-cellular phenotypes, and photosynthesis-inspired energy generation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Photosynthesis: Methods and Protocols aims to aid scientists working toward exciting developments in the understanding and application of photosynthesis.
9781493977864
10.1007/978-1-4939-7786-4 doi