Plant Chemical Genomics Methods and Protocols
Plant Chemical Genomics Methods and Protocols
edited by Friedrich Fauser, Martin Jonikas.
- 1st edition 2018
- 1 recurso en línea (XI, 242 páginas) 60 ilustraciones, 47 ilustraciones a color
- Methods in Molecular Biology 1795 1940-6029 .
Multi-Parametric Screening in Arabidopsis thaliana Seedlings -- Novel Micro-Phenotyping Approach to Chemical Genetic Screening for Increased Plant Tolerance to Abiotic Stress -- Method for Ultra-Rapid High-Content Screening for Biologically Active Chemicals Using Plant Pollen -- A High-Throughput Chemical Screening Method for Inhibitors and Potentiators of Hypersensitive Cell Death using Suspension Cell Culture of Arabidopsis thaliana -- High-Throughput Screening of Chemical Compound Libraries for Modulators of Salicylic Acid Signaling by In Situ Monitoring of Glucuronidase-Based Reporter Gene Expression -- Identification of Chemical Inducers of the Phosphate-Starvation Signaling Pathway in A. thaliana using Chemical Genetics -- Phenotype-Based Screening of Small Molecules to Modify Plant Cell Walls using BY-2 Cells -- Small Molecule Screening to Increase Agrobacterium-Mediated Transformation Efficiency in Legumes -- High-Throughput In Vitro Screening for Inhibitors of Cereal α-glucosidase -- Chemical Screening for Strigolactone Receptor Antagonists using Arabidopsis thaliana -- Chemical Control of ABA Receptors to Enable Plant Protection against Water Stress -- Modes of Action Study of Seed Germination Inhibitor Germostatin by Forward Genetics Screening -- Enantiodifferential Approach for the Target Protein Detection of the Jasmonate Glucoside that Controls the Leaf Closure of Samanea saman -- Using the QCM Biosensor-Based T7 Phage Display Combined with Bioinformatics Analysis for Target Identification of Bioactive Small Molecule -- Genomic Identification and Analysis of Specialized Metabolite Biosynthetic Gene Clusters in Plants using PlantiSMASH -- Chemical Genomics Translatability from Unicellular to Multicellular Models -- Chemical Screening for Flagella-Associated Phenotypes in Chlamydomonas reinhardtii -- FnCpf1-Mediated Targeted Mutagenesis in Plants.
This volume discusses easy-to-follow protocols for researchers in the field of plant chemical genomics. The chapters in this book look at technical workflows of high-throughput chemical compound screens in numerous plant tissues, such as seedlings, roots, and pollen. Chapters also focus on single-celled systems such as Chlamydomonas reinhardtii for chemical screens, and techniques used to modify the target of a small molecule. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Plant Chemical Genomics: Methods and Protocols is a valuable resource to help researchers work with a broad variety of plants for chemical genomics approaches.
9781493978748
10.1007/978-1-4939-7874-8 doi
Multi-Parametric Screening in Arabidopsis thaliana Seedlings -- Novel Micro-Phenotyping Approach to Chemical Genetic Screening for Increased Plant Tolerance to Abiotic Stress -- Method for Ultra-Rapid High-Content Screening for Biologically Active Chemicals Using Plant Pollen -- A High-Throughput Chemical Screening Method for Inhibitors and Potentiators of Hypersensitive Cell Death using Suspension Cell Culture of Arabidopsis thaliana -- High-Throughput Screening of Chemical Compound Libraries for Modulators of Salicylic Acid Signaling by In Situ Monitoring of Glucuronidase-Based Reporter Gene Expression -- Identification of Chemical Inducers of the Phosphate-Starvation Signaling Pathway in A. thaliana using Chemical Genetics -- Phenotype-Based Screening of Small Molecules to Modify Plant Cell Walls using BY-2 Cells -- Small Molecule Screening to Increase Agrobacterium-Mediated Transformation Efficiency in Legumes -- High-Throughput In Vitro Screening for Inhibitors of Cereal α-glucosidase -- Chemical Screening for Strigolactone Receptor Antagonists using Arabidopsis thaliana -- Chemical Control of ABA Receptors to Enable Plant Protection against Water Stress -- Modes of Action Study of Seed Germination Inhibitor Germostatin by Forward Genetics Screening -- Enantiodifferential Approach for the Target Protein Detection of the Jasmonate Glucoside that Controls the Leaf Closure of Samanea saman -- Using the QCM Biosensor-Based T7 Phage Display Combined with Bioinformatics Analysis for Target Identification of Bioactive Small Molecule -- Genomic Identification and Analysis of Specialized Metabolite Biosynthetic Gene Clusters in Plants using PlantiSMASH -- Chemical Genomics Translatability from Unicellular to Multicellular Models -- Chemical Screening for Flagella-Associated Phenotypes in Chlamydomonas reinhardtii -- FnCpf1-Mediated Targeted Mutagenesis in Plants.
This volume discusses easy-to-follow protocols for researchers in the field of plant chemical genomics. The chapters in this book look at technical workflows of high-throughput chemical compound screens in numerous plant tissues, such as seedlings, roots, and pollen. Chapters also focus on single-celled systems such as Chlamydomonas reinhardtii for chemical screens, and techniques used to modify the target of a small molecule. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Plant Chemical Genomics: Methods and Protocols is a valuable resource to help researchers work with a broad variety of plants for chemical genomics approaches.
9781493978748
10.1007/978-1-4939-7874-8 doi