Pulse Field Gel Electrophoresis Methods and Protocols

Pulse Field Gel Electrophoresis Methods and Protocols edited by Kieran Jordan, Marion Dalmasso. - 1st edition 2015 - 1 recurso en línea (XI, 278 páginas) 44 ilustraciones, 20 ilustraciones a color - Methods in Molecular Biology 1301 1940-6029 .

Pulsed-field Gel Electrophoresis for Disease Monitoring and Control -- Harmonization of PFGE Profile Analysis by using Bioinformatics Tools: Example of the Listeria monocytogenes European Union Reference Laboratory Network -- PFGE as a Tool to Track Listeria monocytogenes in Food Processing Facilities: Case Studies -- Case Study of the use of Pulsed Field Gel Electrophoresis in the Detection of a Food-borne Outbreak -- Pulsed-Field Gel Electrophoresis for Listeria monocytogenes -- Pulsed-field Gel Electrophoresis (PFGE) for Pathogenic Cronobacter Species -- Pulsed-Field Gel Electrophoresis of Bacillus cereus Group Strains -- Pulsed-Field Gel Electrophoresis of Staphylococcus aureus -- The Use of Pulsed-Field Gel Electrophoresis for Genotyping of Clostridium difficile -- Molecular Subtyping of Clostridium botulinum by Pulsed-Field Gel Electrophoresis -- Pulsed Field Gel Electrophoresis of Yersinia pestis -- Pulsed Field Gel Electrophoresis of Group A streptococci -- Application of Pulsed Field Gel Electrophoresis to Type Campylobacter jejuni -- Pulsed-field Gel Electrophoresis of Pseudomonas aeruginosa -- PFGE for Shiga toxin-producing Escherichia coli O157:H7 (STEC O157) and non-O157 STEC -- Pulsed Field Gel Electrophoresis (PFGE) of Salmonella enterica --  PFGE Protocols to Distinguish Subspecies of Lactococcus lactis -- The use of PFGE Method in Genotyping of Selected Bacteria Species of the Lactobacillus genus -- Pulsed Field Gel Electrophoresis for Leuconostoc mesenteroides and pseudomesenteroides -- Pulsed Field Gel Electrophoresis for Bifidobacterium -- Pulsed Field Gel Electrophoresis for Dairy Propionibacteria.

This  volume will be of interest to epidemiologists, food microbiologists, and anyone working on comparing bacterial isolates. Pulse Field Gel Electrophoresis: Methods and Protocols guides readers through methods and protocols that will advance the harmonisation of PFGE methodologies and facilitate inter-laboratory comparisons of PFGE profiles from pathogenic and non-pathogenic bacteria. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.   Concise and easy-to-use, Pulse Field Gel Electrophoresis: Methods and Protocols aims to ensure successful results in the further study of this vital field.

9781493925995

10.1007/978-1-4939-2599-5 doi