Methods of Protein Microcharacterization A Practical Handbook
Shively, John E.
Methods of Protein Microcharacterization A Practical Handbook by John E. Shively. - 1st edition 1986 - 1 recurso en línea (XIX, 456 páginas) - Biological Methods .
Microisolation Techniques -- Microisolation of Biologically Active Polypeptides by Reverse-Phase Liquid Chromatography -- Reverse-Phase HPLC Isolation and Microsequence Analysis -- Purification of Microgram Quantities of Proteins by Polyacrylamide Gel Electrophoresis -- Microamino Acid Analysis -- Amino Acid Analysis With Postcolumn Fluorescent Derivatization -- Amino Acid Analysis by o-Phthaldialdehyde Precolumn Derivatization and Reverse-Phase HPLC -- Amino Terminal Analysis -- Manual Edman Sequencing System -- Comparison of the Spinning Cup and Gas-Phase Instruments -- Gas-Phase Protein/Peptide Sequencer -- Design of a Multipurpose Sequencer -- Solid-Phase Methods in Protein Microsequence Analysis -- Analysis of Phenylthiohydantoin Amino Acids by HPLC -- Purification and Preparation of Chemicals for Microsequencing With the Edman Degradation -- Carboxyl-Terminal Analysis -- Microsequence Analysis by Enzymatic Methods -- COOH-Terminal Analysis of Polypeptides by Chemical and Chromatographic Methods -- Mass Spectrometric Analysis of Polypeptides -- Polypeptide Sequence Analysis Using Gas Chromatography-Mass Spectrometry -- Fast Atom Bombardment and Secondary Ion Mass Spectrometry of Peptides and Proteins.
9781592594368
10.1007/978-1-59259-436-8 doi
Methods of Protein Microcharacterization A Practical Handbook by John E. Shively. - 1st edition 1986 - 1 recurso en línea (XIX, 456 páginas) - Biological Methods .
Microisolation Techniques -- Microisolation of Biologically Active Polypeptides by Reverse-Phase Liquid Chromatography -- Reverse-Phase HPLC Isolation and Microsequence Analysis -- Purification of Microgram Quantities of Proteins by Polyacrylamide Gel Electrophoresis -- Microamino Acid Analysis -- Amino Acid Analysis With Postcolumn Fluorescent Derivatization -- Amino Acid Analysis by o-Phthaldialdehyde Precolumn Derivatization and Reverse-Phase HPLC -- Amino Terminal Analysis -- Manual Edman Sequencing System -- Comparison of the Spinning Cup and Gas-Phase Instruments -- Gas-Phase Protein/Peptide Sequencer -- Design of a Multipurpose Sequencer -- Solid-Phase Methods in Protein Microsequence Analysis -- Analysis of Phenylthiohydantoin Amino Acids by HPLC -- Purification and Preparation of Chemicals for Microsequencing With the Edman Degradation -- Carboxyl-Terminal Analysis -- Microsequence Analysis by Enzymatic Methods -- COOH-Terminal Analysis of Polypeptides by Chemical and Chromatographic Methods -- Mass Spectrometric Analysis of Polypeptides -- Polypeptide Sequence Analysis Using Gas Chromatography-Mass Spectrometry -- Fast Atom Bombardment and Secondary Ion Mass Spectrometry of Peptides and Proteins.
9781592594368
10.1007/978-1-59259-436-8 doi