Type-1 Diabetes Methods and Protocols
Type-1 Diabetes Methods and Protocols
edited by Kathleen M. Gillespie.
- 1st edition 2016
- 1 recurso en línea (X, 213 páginas) 44 ilustraciones, 33 ilustraciones a color
- Methods in Molecular Biology 1433 1940-6029 .
Type 1 Diabetes: Prediction And Prevention: Current Perspectives -- The HLA -- Pancreatic Beta Cell Survival and Signaling Pathways: Effects of Type 1 Diabetes-Associated Genetic Variants -- Islet Autoantibody Analysis: Radioimmunoassays -- Islet Autoantibody Analysis: Non-Radioactive Assays - Electrochemiluminescence -- Detection of C-Peptide in Urine as a Measure of Ongoing Beta Cell Function in Type 1 Diabetes -- Histology of the Type 1 Diabetes Pancreas -- Identification of Islet Antigen-Specific CD8 T Cells Using MHCI-Peptide Tetramer Reagents in the Non Obese Diabetic (NOD) Mouse Model of Type 1 Diabetes -- Tracking Immunological Responses of Islet Antigen-Specific T Cells In The Non Obese Diabetic (NOD) Mouse Model Of Type 1 Diabetes -- Adoptive Transfer of Autoimmune Diabetes using Immunodeficient Non Obese Diabetic (NOD) Mice -- Methylation Analysis in Distinct Immune Cell Subsets in Type 1 Diabetes -- FISH with Concomitant Immunofluorescence -- Laser Capture Microdissection: Single Pancreatic Cell DNA Profiling -- The Gut Microbiome in the NOD Mouse -- Molecular Methods and Protein Synthesis for Definition of Autoantibody Epitopes. .
The aim of this volume is to present current methodologies to predict and understand the pathogenesis of Type-1 Diabetes for clinical and non-clinical researchers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Practical and reliable, Type-1 Diabetes: Methods and Protocols will aid researchers in using these methods to advance their own studies.
9781493936434
10.1007/978-1-4939-3643-4 doi
Type 1 Diabetes: Prediction And Prevention: Current Perspectives -- The HLA -- Pancreatic Beta Cell Survival and Signaling Pathways: Effects of Type 1 Diabetes-Associated Genetic Variants -- Islet Autoantibody Analysis: Radioimmunoassays -- Islet Autoantibody Analysis: Non-Radioactive Assays - Electrochemiluminescence -- Detection of C-Peptide in Urine as a Measure of Ongoing Beta Cell Function in Type 1 Diabetes -- Histology of the Type 1 Diabetes Pancreas -- Identification of Islet Antigen-Specific CD8 T Cells Using MHCI-Peptide Tetramer Reagents in the Non Obese Diabetic (NOD) Mouse Model of Type 1 Diabetes -- Tracking Immunological Responses of Islet Antigen-Specific T Cells In The Non Obese Diabetic (NOD) Mouse Model Of Type 1 Diabetes -- Adoptive Transfer of Autoimmune Diabetes using Immunodeficient Non Obese Diabetic (NOD) Mice -- Methylation Analysis in Distinct Immune Cell Subsets in Type 1 Diabetes -- FISH with Concomitant Immunofluorescence -- Laser Capture Microdissection: Single Pancreatic Cell DNA Profiling -- The Gut Microbiome in the NOD Mouse -- Molecular Methods and Protein Synthesis for Definition of Autoantibody Epitopes. .
The aim of this volume is to present current methodologies to predict and understand the pathogenesis of Type-1 Diabetes for clinical and non-clinical researchers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Practical and reliable, Type-1 Diabetes: Methods and Protocols will aid researchers in using these methods to advance their own studies.
9781493936434
10.1007/978-1-4939-3643-4 doi