B Cell Protocols
B Cell Protocols
edited by Hua Gu, Klaus Rajewsky.
- 1st edition 2004
- 1 recurso en línea (XII, 292 páginas)
- Methods in Molecular Biology 271 1940-6029 .
Characterization of B Lymphopoiesis in Mouse Bone Marrow and Spleen -- Characterization of B-Cell Maturation in the Peripheral Immune System -- Identification of B-Cell Subsets -- Analysis of B-Cell Life-Span and Homeostasis -- Differentiation of B Lymphocytes From Hematopoietic Stem Cells -- Analysis of Lymphocyte Development and Function Using the RAG-Deficient Blastocyst Complementation System -- Conditional Gene Mutagenesis in B-Lineage Cells -- Analysis of the Germinal Center Reaction and In Vivo Long-Lived Plasma Cells -- T-Cell-Dependent Immune Responses, Germinal Center Development, and the Analysis of V-Gene Sequences -- of Genes Into Primary Murine Splenic B Cells Using Retrovirus Vectors -- Immunoglobulin Class Switching -- In Vitro and In Vivo Assays of B-Lymphocyte Migration -- Analysis of Antigen-Specific B-Cell Memory Directly Ex Vivo -- B-Cell Signal Transduction -- Isolation of Lipid Rafts From B Lymphocytes -- Molecular Single-Cell PCR Analysis of Rearranged Immunoglobulin Genes As a Tool to Determine the Clonal Composition of Normal and Malignant Human B Cells -- Analysis of B-Cell Immune Tolerance Induction Using Transgenic Mice -- Analysis of B-Cell Signaling Using DT40 B-Cell Line -- B-Cell Development and Pre-B-1 Cell Plasticity In Vitro.
Recent advances in genetics, synergized by many exciting developments in cellular and molecular biology, have provided powerful new experimental tools for the study of not only B-lymphocyte development and function, but also the physiology and pathology of the immune system in higher animals. In B Cell Protocols, highly qualified investigators who are either the inventors or expert users of their methods provide a diverse collection of protocols that range from the latest inventions and applications to classic, but still frequently used, methods in B cell biology. The protocols presented follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, B Cell Protocols offers both beginning and experienced researchers alike highly effective tools for exploring B-lymphocyte development and function in higher animals, as well as critical information on how best to design or modify an experimental approach that will prove productive in their own research.
9781592597963
10.1385/1592597963 doi
Characterization of B Lymphopoiesis in Mouse Bone Marrow and Spleen -- Characterization of B-Cell Maturation in the Peripheral Immune System -- Identification of B-Cell Subsets -- Analysis of B-Cell Life-Span and Homeostasis -- Differentiation of B Lymphocytes From Hematopoietic Stem Cells -- Analysis of Lymphocyte Development and Function Using the RAG-Deficient Blastocyst Complementation System -- Conditional Gene Mutagenesis in B-Lineage Cells -- Analysis of the Germinal Center Reaction and In Vivo Long-Lived Plasma Cells -- T-Cell-Dependent Immune Responses, Germinal Center Development, and the Analysis of V-Gene Sequences -- of Genes Into Primary Murine Splenic B Cells Using Retrovirus Vectors -- Immunoglobulin Class Switching -- In Vitro and In Vivo Assays of B-Lymphocyte Migration -- Analysis of Antigen-Specific B-Cell Memory Directly Ex Vivo -- B-Cell Signal Transduction -- Isolation of Lipid Rafts From B Lymphocytes -- Molecular Single-Cell PCR Analysis of Rearranged Immunoglobulin Genes As a Tool to Determine the Clonal Composition of Normal and Malignant Human B Cells -- Analysis of B-Cell Immune Tolerance Induction Using Transgenic Mice -- Analysis of B-Cell Signaling Using DT40 B-Cell Line -- B-Cell Development and Pre-B-1 Cell Plasticity In Vitro.
Recent advances in genetics, synergized by many exciting developments in cellular and molecular biology, have provided powerful new experimental tools for the study of not only B-lymphocyte development and function, but also the physiology and pathology of the immune system in higher animals. In B Cell Protocols, highly qualified investigators who are either the inventors or expert users of their methods provide a diverse collection of protocols that range from the latest inventions and applications to classic, but still frequently used, methods in B cell biology. The protocols presented follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, B Cell Protocols offers both beginning and experienced researchers alike highly effective tools for exploring B-lymphocyte development and function in higher animals, as well as critical information on how best to design or modify an experimental approach that will prove productive in their own research.
9781592597963
10.1385/1592597963 doi