New Nucleic Acid Techniques
New Nucleic Acid Techniques
edited by John M. Walker.
- 1st edition 1988
- 1 recurso en línea (XVI, 560 páginas) 17 ilustraciones
- Methods in Molecular Biology 4 1940-6029 .
Electrophoresis of RNA Denatured with Glyoxal or Formaldehyde -- Northern Blotting -- Hybrid-Release Translation -- Hybrid-Arrested Translation -- In Vitro Translation and Analysis of Early Events in Protein Synthesis Initiation in Nonreticulocyte Mammalian Cells -- Use of Elutips to Purify DNA -- Plasmid Preparation on Sephacryl S1000 -- Electrophoresis of DNA in Nondenaturing Polyacrylamide Gels -- Use of 35S Nucleotides for DNA Sequencing -- Direct Dideoxy DNA Sequencing -- Computer Applications to Molecular Biology -- Detection of Sequence-Specific Protein-DNA Interactions by the DNA-Footprinting Technique -- Oligonucleotide Synthesis Using the Manual Phosphotriester Method -- Manual Oligonucleotide Synthesis Using the Phosphoramidite Method -- Purification of Synthetic Oligonucleotides by Preparative Gel Electrophoresis -- Isolation of High Molecular Weight DNA Suitable for the Construction of Genomic Libraries -- Construction of Mammalian Genomic Libraries Using ? Replacement Vectors -- Cosmid Library Construction -- Construction of cDNA Libraries in ?gt10 or ?gt11 -- Immunoscreening of ?gt11 Expression Libraries Using an Avidin-Biotin Detection System -- Primed Synthesis and Direct Sequencing in the Isolation of cDNA Clones Using Short Oligonucleotides -- Constructing Expression cDNA Libraries Using Unphosphorylated Adaptors -- Expression Screening of cDNA Libraries in pEX -- Producing Antibodies of Predetermined Specificity from Escherichia coli Hybrid Proteins -- Epitope Mapping Using pEX -- DNA Transfection of Mammalian Cells Using Polybrene -- Expression of Foreign Genes in Mammalian Cells -- Dot-Blot Hybridization Method -- Detection of Unique or Low Copy Number DNA Sequences in Complex Genomes -- Photobiotin-Labeled DNA and RNA Hybridization Probes -- Enzyme-Labeled DNA Probes -- Luminescent Detection of Specific DNA Sequences -- Detection of DNA Sequences Using Biotinylated Probes -- Crosslinking of Single-Stranded DNA to Resins -- Extraction of RNA from Plants -- Isolation of Plant Nuclei -- In Vitro Transcription in Plant Nuclei -- Large-Scale Isolation of Ti Plasmid DNA -- Transformation and Regeneration of Dicotyledonous Plants -- Plant Protoplast Fusion -- Plant Tissue Culture -- Direct Gene Transfer into Plant Protoplasts -- Isolation of Wheat Chloroplasts and Chloroplast DNA -- DNA Labeling by Isolated Wheat Chloroplasts.
9781592594917
10.1385/0896031276 doi
Electrophoresis of RNA Denatured with Glyoxal or Formaldehyde -- Northern Blotting -- Hybrid-Release Translation -- Hybrid-Arrested Translation -- In Vitro Translation and Analysis of Early Events in Protein Synthesis Initiation in Nonreticulocyte Mammalian Cells -- Use of Elutips to Purify DNA -- Plasmid Preparation on Sephacryl S1000 -- Electrophoresis of DNA in Nondenaturing Polyacrylamide Gels -- Use of 35S Nucleotides for DNA Sequencing -- Direct Dideoxy DNA Sequencing -- Computer Applications to Molecular Biology -- Detection of Sequence-Specific Protein-DNA Interactions by the DNA-Footprinting Technique -- Oligonucleotide Synthesis Using the Manual Phosphotriester Method -- Manual Oligonucleotide Synthesis Using the Phosphoramidite Method -- Purification of Synthetic Oligonucleotides by Preparative Gel Electrophoresis -- Isolation of High Molecular Weight DNA Suitable for the Construction of Genomic Libraries -- Construction of Mammalian Genomic Libraries Using ? Replacement Vectors -- Cosmid Library Construction -- Construction of cDNA Libraries in ?gt10 or ?gt11 -- Immunoscreening of ?gt11 Expression Libraries Using an Avidin-Biotin Detection System -- Primed Synthesis and Direct Sequencing in the Isolation of cDNA Clones Using Short Oligonucleotides -- Constructing Expression cDNA Libraries Using Unphosphorylated Adaptors -- Expression Screening of cDNA Libraries in pEX -- Producing Antibodies of Predetermined Specificity from Escherichia coli Hybrid Proteins -- Epitope Mapping Using pEX -- DNA Transfection of Mammalian Cells Using Polybrene -- Expression of Foreign Genes in Mammalian Cells -- Dot-Blot Hybridization Method -- Detection of Unique or Low Copy Number DNA Sequences in Complex Genomes -- Photobiotin-Labeled DNA and RNA Hybridization Probes -- Enzyme-Labeled DNA Probes -- Luminescent Detection of Specific DNA Sequences -- Detection of DNA Sequences Using Biotinylated Probes -- Crosslinking of Single-Stranded DNA to Resins -- Extraction of RNA from Plants -- Isolation of Plant Nuclei -- In Vitro Transcription in Plant Nuclei -- Large-Scale Isolation of Ti Plasmid DNA -- Transformation and Regeneration of Dicotyledonous Plants -- Plant Protoplast Fusion -- Plant Tissue Culture -- Direct Gene Transfer into Plant Protoplasts -- Isolation of Wheat Chloroplasts and Chloroplast DNA -- DNA Labeling by Isolated Wheat Chloroplasts.
9781592594917
10.1385/0896031276 doi