Patch-Clamp Analysis Advanced Techniques
Patch-Clamp Analysis Advanced Techniques
edited by Wolfgang Walz, Alan A. Boulton, Glen B. Baker.
- 1st edition 2002
- 1 recurso en línea (XII, 345 páginas)
- Neuromethods 35 1940-6045 .
Technology of Patch-Clamp Electrodes -- Whole-Cell Patch-Clamp Recordings -- Single-Channel Recording -- Combined Fluorometric and Electrophysiological Recordings -- Patch-Clamp Techniques Applied To Brain Slices -- Perforated Patch-Clamp Technique -- Fast-Drug Application -- Pipet Perfusion Methods -- Patch-Cram Detection of Cyclic GMP in Intact Cells -- Loose-Patch Voltage-Clamp Technique -- Recording from Macropatches -- Combining Patch-Clamp Techniques with RT-PCR.
The use of the patch pipet has moved from a specialized to a mainstream method that can be combined with other techniques to perform complex and novel operations. In Patch-Clamp Analysis: Advanced Techniques, Wolfgang Walz and his colleagues bring together a team of leading academic researchers to describe in reproducible detail all the most important patch-clamp methods. Beginning with updated basic patch-clamp techniques, these hands-on experts present the newest developments, ranging from fast external solution switching and the switching of the pipet solution during recording, to the loose-patch, the perforated-patch, and the patch-cram detection technique. Coverage of these advanced techniques combines their molecular biology and imaging to produce the patch pipet with RT-PCR and fluorometric techniques. All the methods included describe the theoretical background, offer complete startup help, and provide tips on solving common problems and analyzing data. Comprehensive and highly practical, Patch-Clamp Analysis: Advanced Techniques surveys all the major applications that involve patch pipets and sets the stage for the emergence of today's new applications involving genomics, proteomics, and sophisticated imaging techniques.
9781592592760
10.1385/1592592767 doi
Technology of Patch-Clamp Electrodes -- Whole-Cell Patch-Clamp Recordings -- Single-Channel Recording -- Combined Fluorometric and Electrophysiological Recordings -- Patch-Clamp Techniques Applied To Brain Slices -- Perforated Patch-Clamp Technique -- Fast-Drug Application -- Pipet Perfusion Methods -- Patch-Cram Detection of Cyclic GMP in Intact Cells -- Loose-Patch Voltage-Clamp Technique -- Recording from Macropatches -- Combining Patch-Clamp Techniques with RT-PCR.
The use of the patch pipet has moved from a specialized to a mainstream method that can be combined with other techniques to perform complex and novel operations. In Patch-Clamp Analysis: Advanced Techniques, Wolfgang Walz and his colleagues bring together a team of leading academic researchers to describe in reproducible detail all the most important patch-clamp methods. Beginning with updated basic patch-clamp techniques, these hands-on experts present the newest developments, ranging from fast external solution switching and the switching of the pipet solution during recording, to the loose-patch, the perforated-patch, and the patch-cram detection technique. Coverage of these advanced techniques combines their molecular biology and imaging to produce the patch pipet with RT-PCR and fluorometric techniques. All the methods included describe the theoretical background, offer complete startup help, and provide tips on solving common problems and analyzing data. Comprehensive and highly practical, Patch-Clamp Analysis: Advanced Techniques surveys all the major applications that involve patch pipets and sets the stage for the emergence of today's new applications involving genomics, proteomics, and sophisticated imaging techniques.
9781592592760
10.1385/1592592767 doi