New Protein Techniques
New Protein Techniques
edited by John M. Walker.
- 1st edition 1988
- 1 recurso en línea (XV, 531 páginas) 33 ilustraciones
- Methods in Molecular Biology 3 1940-6029 .
Prevention of Unwanted Proteolysis -- The Bradford Method for Protein Quantitation -- Amino Acid Analysis by Precolumn Derivatization -- Identification of N-Terminal Amino Acids by High-Performance Liquid Chromatography -- Enzymatic Methods for Cleaving Proteins -- Chemical Cleavage of Proteins -- Chemical Modification of Proteins -- The Design, Preparation, and Use of Immunopurification Reagents -- Dye-Ligand Chromatography -- Aminohexyl-Sepharose Affinity Chromatography -- Purification of DNA-Dependent RNA Polymerase from Eubacteria -- Direct Immunoprecipitation of Protein -- Detection of Proteins in Polyacrylamide Gels Using an Ultrasensitive Silver Staining Technique -- Chromatofocusing -- Hybrid Isoelectric Focusing Using Mixed Synthetic-Carrier Ampholyte-Immobilized pH Gradient Gels -- Two-Dimensional Electrophoresis Using Immobilized pH Gradients in the First Dimension -- Two-Dimensional Polyacrylamide Gel Electrophoresis Using Flat-Bed Isoelectric Focusing in the First Dimension -- Preparative Aspects of Immobilized pH Gradients -- Isoelectric Focusing Under Denaturing Conditions -- Computer Analysis of 2-D Electrophoresis Gels -- Two-Dimensional (Crossed) Immunoelectrophoresis -- Peptide Synthesis -- Synthesis of a Series of Analogous Peptides Using T-Bags -- Production of Antisera to Synthetic Peptides -- Production of Antibodies Using Proteins in Gel Bands -- Purification of Immunoglobulins Using Protein A-Sepharose -- Antibody-Enzyme Conjugate Formation -- Vacuum Blotting -- Blotting with Plate Electrodes -- Use of Dried Milk for Immunoblotting -- Immunodetection of Proteins on "Western" Blots Using 125I-Labeled Protein A -- Detection of Protein Blots Using the Avidin-Biotin System -- Detection of Protein Blots Using Enzyme-Linked Second Antibodies or Protein A -- Colloidal Gold for the Detection of Proteins on Blots and Immunoblots -- Enzyme Immobilization by Adsorption -- Enzyme Immobilization by Entrapment -- Enzyme Immobilization by Covalent Bonding -- Cell Immobilization.
9781592594900
10.1385/0896031268 doi
Prevention of Unwanted Proteolysis -- The Bradford Method for Protein Quantitation -- Amino Acid Analysis by Precolumn Derivatization -- Identification of N-Terminal Amino Acids by High-Performance Liquid Chromatography -- Enzymatic Methods for Cleaving Proteins -- Chemical Cleavage of Proteins -- Chemical Modification of Proteins -- The Design, Preparation, and Use of Immunopurification Reagents -- Dye-Ligand Chromatography -- Aminohexyl-Sepharose Affinity Chromatography -- Purification of DNA-Dependent RNA Polymerase from Eubacteria -- Direct Immunoprecipitation of Protein -- Detection of Proteins in Polyacrylamide Gels Using an Ultrasensitive Silver Staining Technique -- Chromatofocusing -- Hybrid Isoelectric Focusing Using Mixed Synthetic-Carrier Ampholyte-Immobilized pH Gradient Gels -- Two-Dimensional Electrophoresis Using Immobilized pH Gradients in the First Dimension -- Two-Dimensional Polyacrylamide Gel Electrophoresis Using Flat-Bed Isoelectric Focusing in the First Dimension -- Preparative Aspects of Immobilized pH Gradients -- Isoelectric Focusing Under Denaturing Conditions -- Computer Analysis of 2-D Electrophoresis Gels -- Two-Dimensional (Crossed) Immunoelectrophoresis -- Peptide Synthesis -- Synthesis of a Series of Analogous Peptides Using T-Bags -- Production of Antisera to Synthetic Peptides -- Production of Antibodies Using Proteins in Gel Bands -- Purification of Immunoglobulins Using Protein A-Sepharose -- Antibody-Enzyme Conjugate Formation -- Vacuum Blotting -- Blotting with Plate Electrodes -- Use of Dried Milk for Immunoblotting -- Immunodetection of Proteins on "Western" Blots Using 125I-Labeled Protein A -- Detection of Protein Blots Using the Avidin-Biotin System -- Detection of Protein Blots Using Enzyme-Linked Second Antibodies or Protein A -- Colloidal Gold for the Detection of Proteins on Blots and Immunoblots -- Enzyme Immobilization by Adsorption -- Enzyme Immobilization by Entrapment -- Enzyme Immobilization by Covalent Bonding -- Cell Immobilization.
9781592594900
10.1385/0896031268 doi