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05204nam a22003615i 4500 |
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233984 |
| 003 - IDENTIFICADOR DEL NÚMERO DE CONTROL |
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ES-VaUE |
| 005 - FECHA Y HORA DE LA ÚLTIMA TRANSACCIÓN |
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20221220020534.0 |
| 007 - CAMPO FIJO DE DESCRIPCIÓN FÍSICA--INFORMACIÓN GENERAL |
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cr nn 008mamaa |
| 008 - DATOS DE LONGITUD FIJA--INFORMACIÓN GENERAL |
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100301s2004 xxu| s |||| 0|eng d |
| 020 ## - NÚMERO INTERNACIONAL ESTÁNDAR DEL LIBRO |
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| Número Internacional Estándar del Libro |
9781592597758 |
| 024 7# - IDENTIFICADOR DE OTROS ESTÁNDARES |
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| Número estándar o código |
10.1385/1592597750 |
| Fuente del número o código |
doi |
| 040 ## - FUENTE DE LA CATALOGACIÓN |
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| Centro catalogador/agencia de origen |
ES-MaUEC |
| Lengua de catalogación |
spa |
| Centro/agencia transcriptor |
ES-MaUEC |
| 245 10 - MENCIÓN DE TÍTULO |
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| Título |
RNA Interference, Editing, and Modification |
| Resto del título |
Methods and Protocols |
| Mención de responsabilidad, etc. |
edited by Jonatha M. Gott. |
| 250 ## - MENCIÓN DE EDICIÓN |
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| Mención de edición |
1st edition 2004 |
| 264 #1 - PRODUCCIÓN, PUBLICACIÓN, DISTRIBUCIÓN, FABRICACIÓN Y COPYRIGHT |
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| Producción, publicación, distribución, fabricación y copyright |
Totowa, NJ |
| Nombre del de productor, editor, distribuidor, fabricante |
Humana Press |
| Fecha de producción, publicación, distribución, fabricación o copyright |
2004 |
| 300 ## - DESCRIPCIÓN FÍSICA |
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| Extensión |
1 recurso en línea (XIV, 436 páginas) |
| Otras características físicas |
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| 336 ## - TIPO DE CONTENIDO |
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| Término de tipo de contenido |
texto |
| Código de tipo de contenido |
txt |
| Fuente |
rdacontent |
| 337 ## - TIPO DE MEDIO |
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| Nombre/término del tipo de medio |
electrónico |
| Código del tipo de medio |
c |
| Fuente |
rdamedia |
| 338 ## - TIPO DE SOPORTE |
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| Nombre/término del tipo de soporte |
recurso electrónico |
| Código del tipo de soporte |
cr |
| Fuente |
rdacarrier |
| 347 ## - CARACTERÍSTICAS DEL ARCHIVO DIGITAL |
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| Tipo de archivo |
archivo de texto |
| Formato de codificación |
PDF |
| 490 0# - MENCIÓN DE SERIE |
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| Mención de serie |
Methods in Molecular Biology |
| Número Internacional Normalizado para Publicaciones Seriadas |
1940-6029 |
| Designación de volumen o secuencia |
265 |
| 505 0# - NOTA DE CONTENIDO CON FORMATO |
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| Nota de contenido con formato |
RNA Interference and Gene Silencing -- RNA Interference -- Methods for Delivery of Double-Stranded RNA into Caenorhabditis elegans -- Induction and Biochemical Purification of RNA-Induced Silencing Complex From Drosophila S2 Cells -- Analysis of Gene Function in Trypanosoma brucei Using RNA Interference -- Short Hairpin Activated Gene Silencing in Mammalian Cells -- Geminivirus Vectors for Transient Gene Silencing in Plants -- Posttranscriptional Gene Silencing in Plants -- Identification of microRNAs and Other Tiny Noncoding RNAs by cDNA Cloning -- RNA Editing and Modification -- A Historical Perspective on RNA Editing -- Identification of Substrates for Adenosine Deaminases That Act on RNA -- Purification and Assay of Recombinant ADAR Proteins Expressed in the Yeast Pichia pastoris or in Escherichia coli -- Isolation of an mRNA-Binding Protein Involved in C-to-U Editing -- In Vitro Assays for Kinetoplastid U Insertion-Deletion Editing and Associated Activities -- Identification and Characterization of Trypanosome RNA-Editing Complex Components -- Chimeric Templates and Assays Used to Study Physarum Cotranscriptional Insertional Editing In Vitro -- Methods for Analysis of Mitochondrial tRNA Editing in Acanthamoeba castellanii -- In Vitro RNA Editing Systems From Higher Plant Chloroplasts -- Studying RNA Editing in Transgenic Chloroplasts of Higher Plants -- Detection and Quantification of Modified Nucleotides in RNA Using Thin-Layer Chromatography -- Functional Characterization of 2?-O-Methylation and Pseudouridylation Guide RNAs -- Experimental Rnomics. |
| 520 ## - SUMARIO, ETC. |
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| Sumario, etc. |
The study of RNA interference, editing, and modification has led to major shifts in our understanding of how genes are expressed and regulated. In RNA Interference, Editing, and Modification: Methods and Protocols, hands-on experimentalists describe in detail the protocols and assays they have developed to study these processes in most of the major biological systems in which they are known to occur-a wide range of organisms that includes worms, flies, trypanosomes, mammals, and plants. A historical overview of each subject is provided to put related chapters into a larger context. RNA interference (RNAi) has also emerged in recent years as an important and promising tool for gene discovery and reverse genetic engineering. Application of this cutting-edge technique is described for a range of experimental systems. Topics of interest include stable and transient RNA interference, RNA editing, gene silencing, bioinformatics, small noncoding RNAs, and RNomics. Special attention is given to methods for the identification and characterization of small RNAs, many of which are involved in RNA interference or modification. In addition, techniques used to study RNA editing mechanisms are elaborated for both systems that involve the conversion of one base to another and insertion-deletion editing. To promote the adaptation of their assays and approaches for use in other biological systems, the authors have included a range of methods representative of the major experimental systems in a given field. All protocols presented follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, RNA Interference, Editing, and Modification: Methods and Protocols offers not only geneticists, molecular biologists, and bioinformaticists a comprehensive collection of readily reproducible techniques for the elucidation of gene function and the alteration of gene expression, but also describes how best they may be productively used to address outstanding mechanistic questions and adapted to novel biological systems. |
| 988 ## - NOTA LOCAL 598 |
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| Nota local 598 (boletines) |
Springer_Protocols_2004 |
| 700 1# - PUNTO DE ACCESO ADICIONAL--NOMBRE DE PERSONA |
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| Nombre de persona |
Gott, Jonatha M |
| Término indicativo de función/relación |
editor literario |
| Código de función/relación |
edt |
| -- |
http://id.loc.gov/vocabulary/relators/edt |
| 776 08 - ENTRADA/ENLACE A UN FORMATO FÍSICO ADICIONAL |
|---|
| Información de relación/Frase instructiva de referencia |
Printed edition: |
| Número Internacional Estándar del Libro |
9781617374456 |
| 776 08 - ENTRADA/ENLACE A UN FORMATO FÍSICO ADICIONAL |
|---|
| Información de relación/Frase instructiva de referencia |
Printed edition: |
| Número Internacional Estándar del Libro |
9781588292421 |
| 776 08 - ENTRADA/ENLACE A UN FORMATO FÍSICO ADICIONAL |
|---|
| Información de relación/Frase instructiva de referencia |
Printed edition: |
| Número Internacional Estándar del Libro |
9781489939517 |
| 856 40 - LOCALIZACIÓN Y ACCESO ELECTRÓNICOS |
|---|
| Identificador Uniforme del Recurso |
https://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592597750 |
| Nota pública |
(usuarios Universidad Europea de Valencia) |
| 942 ## - ELEMENTOS DE PUNTO DE ACCESO ADICIONAL (KOHA) |
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| Fuente del sistema de clasificación o colocación |
Library of Congress Classification |
| Tipo de ítem Koha |
LIBRO-E NO PRÉSTAMO |