Detalles MARC
| 000 -CABECERA |
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| campo de control de longitud fija |
06422nam a22003615i 4500 |
| 001 - NÚMERO DE CONTROL |
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| campo de control |
235274 |
| 003 - IDENTIFICADOR DEL NÚMERO DE CONTROL |
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| campo de control |
ES-VaUE |
| 005 - FECHA Y HORA DE LA ÚLTIMA TRANSACCIÓN |
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20221220020655.0 |
| 007 - CAMPO FIJO DE DESCRIPCIÓN FÍSICA--INFORMACIÓN GENERAL |
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| campo de control de longitud fija |
cr nn 008mamaa |
| 008 - DATOS DE LONGITUD FIJA--INFORMACIÓN GENERAL |
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| campo de control de longitud fija |
130228s2013 xxu| s |||| 0|eng d |
| 020 ## - NÚMERO INTERNACIONAL ESTÁNDAR DEL LIBRO |
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| Número Internacional Estándar del Libro |
9781627032841 |
| 024 7# - IDENTIFICADOR DE OTROS ESTÁNDARES |
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| Número estándar o código |
10.1007/978-1-62703-284-1 |
| Fuente del número o código |
doi |
| 040 ## - FUENTE DE LA CATALOGACIÓN |
|---|
| Centro catalogador/agencia de origen |
ES-MaUEC |
| Lengua de catalogación |
spa |
| Centro/agencia transcriptor |
ES-MaUEC |
| 245 10 - MENCIÓN DE TÍTULO |
|---|
| Título |
Gene Regulation |
| Resto del título |
Methods and Protocols |
| Mención de responsabilidad, etc. |
edited by Minou Bina. |
| 250 ## - MENCIÓN DE EDICIÓN |
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| Mención de edición |
1st edition 2013 |
| 264 #1 - PRODUCCIÓN, PUBLICACIÓN, DISTRIBUCIÓN, FABRICACIÓN Y COPYRIGHT |
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| Producción, publicación, distribución, fabricación y copyright |
Totowa, NJ |
| Nombre del de productor, editor, distribuidor, fabricante |
Humana Press |
| Fecha de producción, publicación, distribución, fabricación o copyright |
2013 |
| 300 ## - DESCRIPCIÓN FÍSICA |
|---|
| Extensión |
1 recurso en línea (XV, 401 páginas) |
| Otras características físicas |
80 ilustraciones, 19 ilustraciones a color |
| 336 ## - TIPO DE CONTENIDO |
|---|
| Término de tipo de contenido |
texto |
| Código de tipo de contenido |
txt |
| Fuente |
rdacontent |
| 337 ## - TIPO DE MEDIO |
|---|
| Nombre/término del tipo de medio |
electrónico |
| Código del tipo de medio |
c |
| Fuente |
rdamedia |
| 338 ## - TIPO DE SOPORTE |
|---|
| Nombre/término del tipo de soporte |
recurso electrónico |
| Código del tipo de soporte |
cr |
| Fuente |
rdacarrier |
| 347 ## - CARACTERÍSTICAS DEL ARCHIVO DIGITAL |
|---|
| Tipo de archivo |
archivo de texto |
| Formato de codificación |
PDF |
| 490 0# - MENCIÓN DE SERIE |
|---|
| Mención de serie |
Methods in Molecular Biology |
| Número Internacional Normalizado para Publicaciones Seriadas |
1940-6029 |
| Designación de volumen o secuencia |
977 |
| 505 0# - NOTA DE CONTENIDO CON FORMATO |
|---|
| Nota de contenido con formato |
Gene Regulation -- Isolation of Nuclei for use in Genome-wide DNase Hypersensitivity Assays to Probe Chromatin Structure -- DNase I Digestion of Isolated Nulcei for Genome-Wide Mapping of DNase Hypersensitivity Sites in Chromatin -- Isolation and Analysis of DNA Derived from Nucleosome-Free Regions -- Acquisition of High Quality DNA for Massive Parallel Sequencing by in vivo Chromatin Immunoprecipitation -- Luciferase Assay to Study the Activity of a Cloned Promoter DNA Fragment -- Promoter Deletion Analysis using a Dual-luciferase Reporter System -- Application of mRNA Display for in vitro Selection of DNA-binding Transcription Factor Complexes -- Isolation of Intracellular Protein:DNA Complexes using HaloCHIP; An Antibody Free Alternative to Chromatin Immunoprecipitation -- A Modified Yeast One-Hybrid System for Genome Wide Identification of Transcription Factor Binding Sites -- Identifying Specific Protein--DNA Interactions using SILAC--based Quantitative Proteomics -- Electrophoretic Mobility-Shift and Super-Shift Assays for Studies and Characterization of Protein-DNA Complexes -- Combination of Native and Denaturing PAGE for the Detection of Protein Binding Regions in Long Fragments of Genomic DNA -- Quantitative NanoProteomics Approach for Protein Complex (QNanoPX) using Gold Nanoparticle-based DNA Probe -- Chromatin Assembly and In Vitro Transcription Analyses for Evaluation of Individual Protein Activities in Multicomponent Transcriptional Complexes -- Using FRET to Monitor Protein-induced DNA Bending: The TBP-TATA Complex as a Model System -- Promoter Independent Abortive Transcription Assays Unravel Functional Interactions Between TFIIB and RNA Polymerase -- Fluorescence Cross-correlation Spectroscopy (FCCS) to Observe Dimerization of Transcription Factors in Living Cells -- Nuclear Recruitment Assay as a Tool to validate Transcription Factor Interactions in Mammalian Cells -- Preparation of Cell Lines for Single-Cell Analysis of Transcriptional Activation Dynamics -- Peptide Microarrays for Profiling of Serine/threonine Kinase Activity of Recombinant Kinases and Lysates of Cells and Tissue Samples -- Immunoaffinity Purification of Protein Complexes from Mammalian Cells -- Simple and Efficient Identification of Chromatin Modifying Complexes and Characterization of Complex Composition -- Heavy Methyl-SILAC Labeling Coupled with Liquid Chromatography and High-resolution Mass Spectrometry to Study the Dynamics of Site-specific Histone Methylation -- Analysis of p300 Occupancy at the Early Stage of Stem Cell Differentiation by Chromatin Immunoprecipitation -- Mammalian Two-Hybrid Assays for Studies of Interaction of p300 with Transcription Factors -- Fluorescence Anisotropy Microplate Assay to Investigate the Interaction of Full-length Steroid Receptor Coactivator-1a with Steroid Receptors -- Use of Histone Deacetylase Inhibitors to Examine the Roles of Bromodomain and Histone Acetylation in p300-dependent Gene Expression -- Histone Deacetylases Valproic Acid as a Small Molecule Inducer to Direct the Differentiation of Pluripotent Stem Cells -- Sedimentation and Immunoprecipitation Assays for Analyzing Complexes that Repress Transcription -- Methods for Studies of Protein Interactions with Different DNA Methyltransferases. |
| 520 ## - SUMARIO, ETC. |
|---|
| Sumario, etc. |
In this volume of Methods in Molecular Biology™, expert investigators offer comprehensive, complementary, and cutting-edge technologies for studies of gene regulation. The chapters of Gene Regulation: Methods and Protocols are organized to provide an integrated and a coherent view of control systems and their associated components. The protocols are broad in their scope. They include molecular, biochemical, spectroscopic techniques as well as high throughput strategies. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Comprehensive and broad in their scope, the protocols are useful to researchers in many disciplines including molecular biology, genomics, biochemistry, biomedicine, nutrition, and agricultural sciences. FEATURES • Techniques for studying chromatin structural features that influence gene activation • In vivo and in vitro assays • Spectroscopic techniques • Technologies for examining genome-wide association of transcription factors with regulatory regions of genes • Proteomics based on SILAC (Stable Isotope Labeling of Amino Acids in Cell Culture) • Peptide microarray technology for studies of kinases • Schemes for isolation and characterization of relatively large multiprotein complexes • Biochemical and biophysical techniques for examining gene repression • In vitro chromatin reconstitution to investigate multicomponent transcriptional complexes • Nuclear recruitment assay to validate transcription factor interactions in mammalian cells. |
| 988 ## - NOTA LOCAL 598 |
|---|
| Nota local 598 (boletines) |
Springer_Protocols_2013 |
| 700 1# - PUNTO DE ACCESO ADICIONAL--NOMBRE DE PERSONA |
|---|
| Nombre de persona |
Bina, Minou |
| Término indicativo de función/relación |
editor literario |
| Código de función/relación |
edt |
| -- |
http://id.loc.gov/vocabulary/relators/edt |
| 776 08 - ENTRADA/ENLACE A UN FORMATO FÍSICO ADICIONAL |
|---|
| Información de relación/Frase instructiva de referencia |
Printed edition: |
| Número Internacional Estándar del Libro |
9781627032858 |
| 776 08 - ENTRADA/ENLACE A UN FORMATO FÍSICO ADICIONAL |
|---|
| Información de relación/Frase instructiva de referencia |
Printed edition: |
| Número Internacional Estándar del Libro |
9781627032834 |
| 776 08 - ENTRADA/ENLACE A UN FORMATO FÍSICO ADICIONAL |
|---|
| Información de relación/Frase instructiva de referencia |
Printed edition: |
| Número Internacional Estándar del Libro |
9781493962884 |
| 856 40 - LOCALIZACIÓN Y ACCESO ELECTRÓNICOS |
|---|
| Identificador Uniforme del Recurso |
https://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-284-1 |
| Nota pública |
(usuarios Universidad Europea de Valencia) |
| 942 ## - ELEMENTOS DE PUNTO DE ACCESO ADICIONAL (KOHA) |
|---|
| Fuente del sistema de clasificación o colocación |
Library of Congress Classification |
| Tipo de ítem Koha |
LIBRO-E NO PRÉSTAMO |