Detalles MARC
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05981nam a22003615i 4500 |
| 001 - NÚMERO DE CONTROL |
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235493 |
| 003 - IDENTIFICADOR DEL NÚMERO DE CONTROL |
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ES-VaUE |
| 005 - FECHA Y HORA DE LA ÚLTIMA TRANSACCIÓN |
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20221220020708.0 |
| 007 - CAMPO FIJO DE DESCRIPCIÓN FÍSICA--INFORMACIÓN GENERAL |
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cr nn 008mamaa |
| 008 - DATOS DE LONGITUD FIJA--INFORMACIÓN GENERAL |
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| campo de control de longitud fija |
100301s2004 xxu| s |||| 0|eng d |
| 020 ## - NÚMERO INTERNACIONAL ESTÁNDAR DEL LIBRO |
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| Número Internacional Estándar del Libro |
9781592597628 |
| 024 7# - IDENTIFICADOR DE OTROS ESTÁNDARES |
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| Número estándar o código |
10.1385/1592597629 |
| Fuente del número o código |
doi |
| 040 ## - FUENTE DE LA CATALOGACIÓN |
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| Centro catalogador/agencia de origen |
ES-MaUEC |
| Lengua de catalogación |
spa |
| Centro/agencia transcriptor |
ES-MaUEC |
| 245 10 - MENCIÓN DE TÍTULO |
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| Título |
Protein'Protein Interactions |
| Resto del título |
Methods and Applications |
| Mención de responsabilidad, etc. |
edited by Haian Fu. |
| 250 ## - MENCIÓN DE EDICIÓN |
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| Mención de edición |
1st edition 2004 |
| 264 #1 - PRODUCCIÓN, PUBLICACIÓN, DISTRIBUCIÓN, FABRICACIÓN Y COPYRIGHT |
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| Producción, publicación, distribución, fabricación y copyright |
Totowa, NJ |
| Nombre del de productor, editor, distribuidor, fabricante |
Humana Press |
| Fecha de producción, publicación, distribución, fabricación o copyright |
2004 |
| 300 ## - DESCRIPCIÓN FÍSICA |
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| Extensión |
1 recurso en línea (XVI, 532 páginas) |
| Otras características físicas |
|
| 336 ## - TIPO DE CONTENIDO |
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| Término de tipo de contenido |
texto |
| Código de tipo de contenido |
txt |
| Fuente |
rdacontent |
| 337 ## - TIPO DE MEDIO |
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| Nombre/término del tipo de medio |
electrónico |
| Código del tipo de medio |
c |
| Fuente |
rdamedia |
| 338 ## - TIPO DE SOPORTE |
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| Nombre/término del tipo de soporte |
recurso electrónico |
| Código del tipo de soporte |
cr |
| Fuente |
rdacarrier |
| 347 ## - CARACTERÍSTICAS DEL ARCHIVO DIGITAL |
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| Tipo de archivo |
archivo de texto |
| Formato de codificación |
PDF |
| 490 0# - MENCIÓN DE SERIE |
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| Mención de serie |
Methods in Molecular Biology |
| Número Internacional Normalizado para Publicaciones Seriadas |
1940-6029 |
| Designación de volumen o secuencia |
261 |
| 505 0# - NOTA DE CONTENIDO CON FORMATO |
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| Nota de contenido con formato |
Overview -- Structural Basis of Protein-Protein Interactions -- Quantitative Analysis of Protein-Protein Interactions -- In Vitro Techniques -- Characterization of Protein-Protein Interactions by Isothermal Titration Calorimetry -- Circular Dichroism Analysis for Protein-Protein Interactions -- Protein-Protein Interaction Analysis by Nuclear Magnetic Resonance Spectroscopy -- Measuring Rhodopsin-G-Protein Interactions by Surface Plasmon Resonance -- Using Light Scattering to Determine the Stoichiometry of Protein Complexes -- Sedimentation Equilibrium Studies -- Analysis of Protein-Protein Interactions by Simulation of Small-Zone Gel Filtration Chromatography -- Fluorescence Gel Retardation Assay to Detect Protein-Protein Interactions -- Fluorescence Polarization Assay to Quantify Protein-Protein Interactions -- Studying Protein-Protein Interactions via Blot Overlay or Far Western Blot -- Glutathione-S-Transferase-Fusion Based Assays for Studying Protein-Protein Interactions -- Affinity Capillary Electrophoresis Analyses of Protein-Protein Interactions in Target-Directed Drug Discovery -- Mapping Protein-Ligand Interactions by Hydroxyl-Radical Protein Footprinting -- Use of Phage Display and Polyvalency to Design Inhibitors of Protein-Protein Interactions -- Detecting Protein-Protein Interactions in Heterologous Systems -- A Bacterial Two-Hybrid System Based on Transcription Activation -- Using the Yeast Two-Hybrid System to Identify Interacting Proteins -- Analysis of Protein-Protein Interactions Utilizing Dual Bait Yeast Two-Hybrid System -- The Split-Ubiquitin Membrane-Based Yeast Two-Hybrid System -- Reverse Two-Hybrid Techniques in the Yeast Saccharomyces cerevisiae -- Mammalian Two-Hybrid Assay for Detecting Protein-Protein Interactions In Vivo -- Co-Immunoprecipitation from Transfected Cells -- Probing Protein-Protein Interactions in Living Cells -- Microscopic Analysis of Fluorescence Resonance Energy Transfer (FRET) -- Monitoring Molecular Interactions in Living Cells Using Flow Cytometric Analysis of Fluorescence Resonance Energy Transfer -- Fluorescence Correlation Spectroscopy -- Confocal Microscopy for Intracellular Co-Localization of Proteins -- Mapping Biochemical Networks With Protein-Fragment Complementation Assays -- In Vivo Protein Cross-Linking -- Proteomics-Based Approaches -- Computational Prediction of Protein-Protein Interactions -- Affinity Methods for Phosphorylation-Dependent Interactions -- Two-Dimensional Gel Electrophoresis for Analysis of Protein Complexes -- Sample Preparation of Gel Electrophoretically Separated Protein Binding Partners for Analysis by Mass Spectrometry -- Quantitative Protein Analysis by Solid Phase Isotope Tagging and Mass Spectrometry -- Internet Resources for Studying Protein-Protein Interactions. |
| 520 ## - SUMARIO, ETC. |
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| Sumario, etc. |
Proteins continuously interact with each other to determine cell fate. Consequently, an examination of just when such protein-protein interactions occur and how they are controlled is essential for understanding the molecular mechanism of biological processes, elucidating the molecular basis of diseases, and identifying potential targets for therapeutic interventions. In Protein-Protein Interactions: Methods and Applications, leading experts describe in detail their highly successful biochemical, biophysical, genetic, and computational techniques for studying these interactions. Their readily reproducible methods demonstrate how to identify protein interaction partners, qualitatively or quantitatively measure protein-protein interactions, monitor protein-protein interactions as they occur in living cells, and determine interaction interfaces. The techniques described utilize a variety of cutting-edge technologies, including surface plasmon resonance (SRP), fluorescence resonance energy transfer (FRET), fluorescence polarization (FP), isothermal titration calorimetry (ITC), circular dichroism (CD), protein fragment complementation assays (PCA), various two-hybrid systems, and proteomics- and bioinformatics-based approaches, such as the Scansite program for computational analysis. Each time-tested protocol includes a background introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Authoritative and highly practical, Protein-Protein Interactions: Methods and Applications offers both beginning and experienced investigators a full range of the powerful tools needed for deciphering how proteins interact to form biological networks, as well as for unraveling protein-protein interactions in disease in the search for novel therapeutic targets. |
| 988 ## - NOTA LOCAL 598 |
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| Nota local 598 (boletines) |
Springer_Protocols_2004 |
| 700 1# - PUNTO DE ACCESO ADICIONAL--NOMBRE DE PERSONA |
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| Nombre de persona |
Fu, Haian |
| Término indicativo de función/relación |
editor literario |
| Código de función/relación |
edt |
| -- |
http://id.loc.gov/vocabulary/relators/edt |
| 776 08 - ENTRADA/ENLACE A UN FORMATO FÍSICO ADICIONAL |
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| Información de relación/Frase instructiva de referencia |
Printed edition: |
| Número Internacional Estándar del Libro |
9781617373732 |
| 776 08 - ENTRADA/ENLACE A UN FORMATO FÍSICO ADICIONAL |
|---|
| Información de relación/Frase instructiva de referencia |
Printed edition: |
| Número Internacional Estándar del Libro |
9781489939685 |
| 776 08 - ENTRADA/ENLACE A UN FORMATO FÍSICO ADICIONAL |
|---|
| Información de relación/Frase instructiva de referencia |
Printed edition: |
| Número Internacional Estándar del Libro |
9781588291202 |
| 856 40 - LOCALIZACIÓN Y ACCESO ELECTRÓNICOS |
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| Identificador Uniforme del Recurso |
https://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592597629 |
| Nota pública |
(usuarios Universidad Europea de Valencia) |
| 942 ## - ELEMENTOS DE PUNTO DE ACCESO ADICIONAL (KOHA) |
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| Fuente del sistema de clasificación o colocación |
Library of Congress Classification |
| Tipo de ítem Koha |
LIBRO-E NO PRÉSTAMO |