Introduction to RNA Vaccines -- Self-replicating RNA -- Self-replicating RNA Vaccine Delivery to Dendritic Cells -- Plant Expression of Trans-encapsidated Viral Nanoparticle Vaccines with Animal RNA Replicons -- RNActive® Technology: Generation and Testing of Stable and Immunogenic mRNA Vaccines -- Nucleoside Modified mRNA Vaccines for Infectious Diseases -- Generation and Evaluation of Prophylactic mRNA Vaccines against Allergy -- Measuring the Adjuvant Activity of RNA Vaccines -- Generation of Immunostimulating 130 nm Protamine-RNA Nanoparticles -- Electroporation of mRNA as Universal Technology Platform to Transfect a Variety of Primary Cells with Antigens and Functional Proteins -- Adjuvant-Enhanced mRNA Vaccines -- Enhanced Delivery of DNA or RNA Vaccines by Electroporation -- The European Regulatory Environment of RNA-Based Vaccines -- Discovery and Subtyping of Neo-Epitope Specific T-cell Responses for Cancer Immunotherapy: Addressing the Mutanome -- Considerations for Producing mRNA Vaccines for Clinical Trials -- Nonclinical Safety Testing of RNA Vaccines -- Immunotherapy of Uveal Melanoma: Vaccination Against Cancer.

This volume provides an overview of the field and practical hints for vaccinologists in academia and industry. Chapters provide protocols on self-replicating RNA vectors, non-replication mRNA vectors, adjuvantation and delivery, and preclinical and clinical development. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNA Vaccines: Methods and Protocols aims to increased collaboration on RNA vaccines between basic and applied scientists in academia, government, and industry to develop future solutions for today's challenges.