SILAC-Based Comparative Proteomic Analysis of Lysosomes from Mammalian Cells Using LC-MS/MS -- Quantitative Profiling of Lysosomal Lipidome by Shotgun Lipidomics Analysis of N- And O-Glycosylation of Lysosomal Glycoproteins -- Analyzing Lysosome Related Organelles By Electron Microscopy -- Microscopic Analysis of Lysosomal Membrane Permeabilization -- Quantitative Co-localization and Pattern Analysis Of Endo-Lysosomal Cargo in Subcellular Image Cytometry and Validation on Synthetic Image Sets -- Preparation of a Two-Photon Fluorescent Probe for Imaging H2O2 in Lysosomes in Living Cells and Tissues -- Lysophagy: A Method for Monitoring Lysosomal Rupture Followed by Autophagy Dependent Recovery -- Delivery of Cargo to Lysosomes using Gneosomes -- Lysosomal Acidification in Cultured Astrocytes Using Nanoparticles -- Analysis of Lysosomal pH by Flow Cytometry Using FITC-dextran Loaded Cells -- Detection of Lysosomal Exocytosis in Platelets by Flow Cytometry -- Detection of Lysosomal Exocytosis by Surface Exposure of Lamp1 Luminal Epitopes -- Using the MEROPS Database for Investigation of Lysosomal Peptidases, their Inhibitors and Substrates -- Next Generation Sequencing Approaches to Define the Role of the Autophagy Lysosomal Pathway in Human Disease: The Example of Lysoplex -- Gelatin Zymography using Leupeptin for the Detection of Various Cathepsin L Forms -- Methods for Determination of Α-Glycosidase, Β-Glycosidase, and Α-Galactosidase Activities in Dried Blood Spot Samples -- Prenatal Diagnosis of Lysosomal Storage Disorders Using Chorionic Villi -- Lysosomal Biology in Cancer. .

This volume provides laboratory protocols essential for studies on lysosomal biology. Chapters aim to guide researchers in their exploration of lysosomes, both under normal conditions and in pathological processes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Lysosome: Methods and Protocols aims to provided protocols that will guide and inspire further research and generate new insights into this fascinating organelle.