Synthesis of Cationic Polymer Libraries for Gene Delivery using Diglycidyl Ethers -- Generation of Ultra-Small PLGA Nanoparticles by Sequential Centrifugation -- Construction of Bacteria-Based Cargo Carriers for Targeted Cancer Therapy -- Production of Extracellular Vesicles Loaded with Therapeutic Cargo -- Delivery of Cytotoxic Mesenchymal Stem Cells with Biodegradable Scaffolds for Treatment of Postoperative Brain Cancer -- Nanoparticles for Targeted Drug Delivery to Cancer Stem-Like Cells and Tumor -- Exploiting Phage Display for Development of Novel Cellular Targeting Strategies -- CD44 Targeted Lipid Nanoparticles for MicroRNA Therapy -- Ultrasound and Microbubble-Mediated Blood-Brain Barrier Disruption for Targeted Delivery of Therapeutics to the Brain -- In Vitro Validation of Targeting and Comparison to Mathematical Modeling -- Enzyme-Linked Immunosorbent Assay to Quantify Targeting Molecules on Nanoparticles -- Tunable Collagen Microfluidic Platform to Study Nanoparticle Transport in the Tumor Microenvironment -- Utilizing the Lung as a Model to Study Nanoparticle Based Drug Delivery Systems -- Non-Enzymatic Tissue Homogenization for Biodistribution Analysis -- Methods and Study Designs for Characterizing the Pharmacokinetics and Pharmacodynamics of Carrier-Mediated Agents.

This volume looks at the latest techniques used to enable therapeutic molecules to be targeted for site-specific delivery. The chapters in this book discuss topics such as approaches to express biologically derived and synthetic nanocarriers; strategies to assist in nanocarrier targeting to specific cells and tissues; and methods for evaluating delivery and efficiency for new classes of agents. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Targeted Drug Delivery: Methods and Protocols is a valuable resource for anyone interested in understanding the diversity of methods used in this evolving field.