Part 1. Overview of Lipases, Phospholipases and Esterases -- Lipases: An Overview -- Carbohydrate Esterases: An Overview -- Phospholipases: An Overview -- Part 2. Activity Screening and Expression -- Functional-Based Screening Methods for Detecting Esterase and Lipase Activity Against Multiple Substrates -- A Continuous and Sensitive Spectrophotometric Assay for Lipase and Phospholipase A Activities using α-Eleostearic Acid-Containing Substrates -- Direct and Continuous Measurement of Phospholipase D Activities Using the Chelation-Enhanced Fluorescence Property of 8-Hydroxyquinoline -- Lipase and Phospholipase Activity Methods for Marine Organisms -- Plant Lipases: Functional Expression of CPLIP1 from Carica papaya -- Heterologous Expression and Functional Characterization of Sparidae Fish Digestive Phospholipase A2 -- Expression and Purification of Recombinant Vigna unguiculata Phospholipase D in Pichia pastoris for Structural Studies -- Part 3. Optimization of the Biocatalyst Production and Performances -- Recent Advances in Pichia pastoris as Host for Heterologous Expression Systems for Lipases (Review) -- Solid-State Fermentation as an Economic Production Method of Lipases and Esterases -- Synthetic Biology to Improve the Production of Lipases and Esterases (Review) -- Rational Design Strategy as a Novel Immobilization Methodology Applied to Lipases and Phospholipases -- Part 4. Applications of Lipases, Phospholipases and Esterases -- Lipase-Catalyzed Synthesis of Fatty Acid Esters of Trisaccharides -- Targeting Phospholipase D genetically and Pharmacologically for Studying Leukocyte Function -- Lipase-Catalyzed Synthesis of Structured Lipids -- Lipase-Catalyzed Acetylation and Esterification of Bile Acids -- Lipases in Green Chemistry: Deep Eutectic Solvents (DES) as New Green Solvents -- Chemoenzymatic Synthesis of Nitrogen Polymers with Biomedical Applications Catalyzed by Lipases -- Lipases and Esterases as Biocatalyst for Biodiesel Production (Review) -- Lipases, Phospholipases and Esterases Biosensors (Review).

This volume provides an essential update on fundamental issues, current and new applications, as well as practical protocols to explore the extensive applications of lipases and the potential application of phospholipases. After an overview, the book delves into activity screening and expression, optimization of the biocatalyst production and performances, and applications of lipases, phospholipases, and esterases. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Lipases and Phospholipases: Methods and Protocols, Second Edition serves as an updated reference book for the large scientific community, both seasoned and novice, working with lipases, phospholipases, and related enzymes.