SEA Ligation is Accelerated at Midly Acidic pH: Application to the Formation of Difficult Peptide Junctions -- The Problem of Aspartimide Formation during Protein Chemical Synthesis Using SEA-Mediated Ligation -- Using the Interactive Tool of the Protein Chemical Synthesis Database -- Only a "Click" Away: Development of Arginine-Rich Peptide-Based Materials Using Click Chemistry -- Fluoroglycoproteins by Copper-Free Strain-Promoted Azide-Alkyne Cycloaddition -- Hybrid Silylated Peptides for the Design of Bio-Functionalized Materials -- Synthesis of Peptide-Oligoethylene Glycol (OEG) Conjugates for Multivalent Modification of Nanomaterials -- Phage Display Methodologies -- Whole-Bacterium Ribosome Display Selection for Isolation of Anti-Bacterial Affitins -- CIS Display: DNA-Based Technology as a Platform for Discovery of Therapeutic Biologics -- Building Scarless Gene Libraries in the Chromosome of Bacteria -- Streptavidin (Sav)-Based Artificial Metalloenzymes: Cofactor Design Considerations and Large Scale Expression of Host Protein Variants -- Integrated Bacterial Production of Large Combinatorial Libraries of Cyclic Oligopeptides and Functional Screening for the Identification of Chemical Rescuers of Pathogenic Protein Misfolding and Aggregation -- Site-Specific Incorporation of Non-Canonical Amino Acids by Amber Stop Codon Suppression in Escherichia coli.

This thorough book aims to present the methods that have enabled the success of peptides and proteins in a wide variety of applications. It opens with a section on chemical tools applied to the production or engineering of peptides and proteins, and concludes with a collection of chapters on biological approaches used to engineer structure and function in peptides and proteins. As a book in the Springer Protocols Handbooks series, chapters include the kind of detailed descriptions and tips necessary for successful results in practice. Authoritative and practical, Peptide and Protein Engineering: From Concepts to Biotechnological Applications will be of great use to scientists in academia and industry seeking a better understanding of the emerging principles and methodologies in peptide and protein engineering.