Application of Neutralization and Freeze-Drying Technique for the Preparation of the Beneficially in the Drug Delivery 2-Hydroxypropyl-β-Cyclodextrin Complexes with Bioactive Molecules -- Functionalized Carbon Nanohorns as Drug Delivery Platforms -- Ultrasonics-Assisted Effective Isolation and Characterization of Exosomes from Whole Organs -- Aggregate Determination by Permeation Technique -- Study of Candesartan Cilexitil:2-Hydroxypropyl-β-Cyclodextrin Interactions: A Computational Approach Using Steered Molecular Dynamics Simulations -- Drug Delivery: Hydrophobic Drug Encapsulation into Amphiphilic Block Copolymer Micelles -- Multisensitive Polymeric Nanocontainers as Drug Delivery Systems: Biological Evaluation -- Drug Incorporation in the Drug Delivery System of Micelles -- Molecular Dynamics Protocols for the Study of Cyclodextrin Drug Delivery Systems -- Drug Delivery through Multifunctional Polypeptidic Hydrogels -- Polymersomes from Hybrids-Polypeptides for Drug Delivery Applications -- Drug Delivery Systems Based on Modified Polysaccharides: Synthesis and Characterization -- Differential Scanning Calorimetry (DSC) on Sartan/Cyclodextrin Delivery Formulations -- Encapsulation of Small Drugs in a Supramolecule Enhances Solubility, Stability, and Therapeutic Efficacy against Glioblastoma Multiforme -- Unveiling the Thermodynamic Aspects of Drug-Cyclodextrin Interactions through Isothermal Titration Calorimetry -- Antitumor Efficacy of Ceranib-2 with Nano-Formulation of PEG and Rosin Esters -- Association of the Thermodynamics with the Functionality of Thermoresponsive Chimeric Nanosystems -- 2D DOSY NMR: A Valuable Tool to Confirm the Complexation in Drug Delivery Systems -- Drug-Encapsulated Cyclodextrin Nanosponges -- Electrochemistry Investigation of Drugs Encapsulated in Cyclodextrins -- A Differential Scanning Calorimetry (DSC) Experimental Protocol for Evaluating the Modified Thermotropic Behavior of Liposomes with Incorporated Guest Molecules -- Applications of NMR in Drug:Cyclodextrin Complexes -- Construction of Peptide-Drug Conjugates for Selective Targeting of Malignant Tumor Cells.

This detailed book aims to provide readers with critical information to accomplish the synthesis of nanosystems for the purpose of supramolecular entities complexing with drugs, targeted drug delivery system characterization, as well as the study of the physical-chemical interactions that govern the stability and properties of these systems. Beginning with a collection of chapters on drug delivery platforms such as cyclodextrins, micelles, liposomes, polymeric, nanotubes, and more, the volume continues with coverage of the study of nanotechnology systems using different biophysical techniques such as DSC, ITC, solid and liquid NMR spectroscopy, and electrochemistry. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Supramolecules in Drug Discovery and Drug Delivery: Methods and Protocols serves as an ideal guide for researchers working toward drug delivery mechanisms that can tailor their physical chemical properties and enhance their efficacy, while retaining their structures intact.