Axon Degeneration: Which Method to Choose? -- Axon Degeneration Assays in Superior Cervical Ganglion Explant Cultures -- Microinjection of Superior Cervical Ganglion Neurons for Studying Axon Degeneration -- Assessing Axonal Degeneration in Embryonic Dorsal Root Ganglion Neurons In Vitro -- Viral Transduction of DRG Neurons -- Planning and Analysis of Axon Degeneration Screening Experiments -- A Microfluidic Culture Platform to Assess Axon Degeneration -- A Schwann Cell-Neuron Co-Culture System to Study Neuron-Glia Interaction during Axon Degeneration -- Establishing Myelinating Co-Cultures Using Human iPSC-Derived Sensory Neurons to Investigate Axonal Degeneration and Demyelination -- Preparation of Organotypic Hippocampal Slice Cultures for the Study of CNS Disease and Damage -- Organotypic Culture Assay for Neuromuscular Synaptic Degeneration and Function -- Intracellular Recordings of Postsynaptic Voltage Responses at the Drosophila Neuromuscular Junction -- Ex Vivo Studies of Optic Nerve Axon Electrophysiology -- Ex Vivo Analysis of Axonal Degeneration Using Sciatic and Optic Nerve Preparations -- Measuring Bioenergetic Signatures of Peripheral Nerve Segments by Extracellular Flux Analysis -- Detection of Neutrophils in the Sciatic Nerve Following Peripheral Nerve Injury -- Whole-Tissue Immunolabeling and 3D Fluorescence Imaging to Visualize Axon Degeneration in the Intact, Unsectioned Mouse Tissues -- Transmission Electron Microscopy and Morphometry of the CNS White Matter -- In Vivo Calcium Imaging During Axon Degeneration in Zebrafish -- In Vivo Imaging of Anterograde and Retrograde Axonal Transport in Rodent Peripheral Nerves -- In Vivo Visualization of Moving Synaptic Cargo Complexes within Drosophila Larval Segmental Axons -- A Chemotherapy-Induced Peripheral Neuropathy Model in Drosophila melanogaster -- Models of Axon Degeneration in Drosophila Larvae -- In Vivo Analysis of Glial Immune Responses to Axon Degeneration in Drosophila melanogaster.

This book is a collection of classical as well as innovative methods used to investigate axon degeneration with a particular focus on addressing the common challenges encountered while performing these procedures. Particular attention is devoted to the study of axon loss in several model organisms, as each poses unique challenges and provides powerful advantages. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Axon Degeneration: Methods and Protocols is an ideal guide for facilitating the application and further development of these protocols, which will help the scientific community tackle important questions regarding axon degeneration. Chapters 2, 3, and 20 are available Open Access under a Creative Commons Attribution 4.0 International License via link.springer.com.