Introduction: The Use of Transgenic Mice in Biomedical Research -- Genetic Modification of the Mouse -- In Vitro Fertilization/Cryopreservation -- Transgene Design -- Generating Conditional Mouse Mutants via Tetracycline-Controlled Gene Expression -- Manipulating Large Insert Clones for Transgenesis -- Reporter Molecules in Genetically Engineered Mice -- Gene Targeting in Mouse Embryonic Stem Cells -- Conditional Knockout Mice -- Knockin Approaches -- Aggregation of Embryos and Embryonic Stem Cells -- Andenovirus-Mediated Gene Transfer -- Random ENU Mutagenesis -- Autopsy and Histologic Analysis of the Transgenic Mouse -- Bone Marrow Transplantations to Study Gene Function in Hematopoietic Cells -- Quantification of Atherosclerosis in Mice -- Genetically Modified Mice in Cancer Research -- Neuropathobiology in Transgenic Mice.

Genetically very similar to the human species, mice play an important role in biomedical research and have served as experimental models for a wide variety of pathologies, including cancer, cardiovascular diseases, and behavioral disorders. In Transgenic Mouse Methods and Protocols, Marten Hofker and Jan van Deursen have assembled a multidisciplinary collection of readily reproducible methods for working with mice, and particularly for generating mouse models that will enable us to better understand gene function. Described in step-by-step detail by highly experienced investigators, these proven techniques include new methods for conditional, induced knockout, and transgenic mice, as well as for working with mice in such important research areas as immunology, cancer, and atherosclerosis. Such alternative strategies as random mutagenesis and viral gene transduction for studying gene function in the mouse are also presented. Care is taken to make clear the details of the available approaches, as well as their limitations. Up-to-date and highly practical, Transgenic Mouse Methods and Protocols demonstrates clearly for both novice and expert investigators how to make novel transgenic mouse models, and how to use them effectively to understand the role of gene function in human health and disease.