Current Trends in Liposome Research -- Nanoliposomes: Preparation and Analysis -- Preparation of DRV Liposomes -- Elastic Liposomes for Topical and Transdermal Drug Delivery -- Archaebacterial Tetraetherlipid Liposomes -- Cationic Magnetoliposomes -- Ultrasound-Responsive Liposomes -- Liposome Formulations of Hydrophobic Drugs -- Remote Loading of Anthracyclines into Liposomes -- Arsonoliposomes: Preparation and Physicochemical Characterization -- Liposome-Based Vaccines -- Mannosylated Liposomes for Targeted Vaccines Delivery -- Liposomes for Specific Depletion of Macrophages from Organs and Tissues -- Vesicular Phospholipid Gels -- Environment-Responsive Multifunctional Liposomes -- Functional Liposomal Membranes for Triggered Release -- A "Dock and Lock" Approach to Preparation of Targeted Liposomes -- Conjugation of Ligands to the Surface of Preformed Liposomes by Click Chemistry -- Targeted Magnetic Liposomes Loaded with Doxorubicin -- Liposomes for Drug Delivery to Mitochondria -- Cytoskeletal-Antigen Specific Immunoliposomes: Preservation of Myocardial Viability -- Gadolinium-Loaded Polychelating Polymer-Containing Tumor-Targeted Liposomes -- Angiogenic Vessel-Targeting DDS by Liposomalized Oligopeptides -- TAT-Peptide Modified Liposomes: Preparation, Characterization, and Cellular Interaction -- ATP-Loaded Liposomes for Targeted Treatment in Models of Myocardial Ischemia -- Intracellular ATP Delivery Using Highly Fusogenic Liposomes -- Lipoplex Formation Using Liposomes Prepared by Ethanol Injection -- Acid-Labile Liposome/pDNA Complexes -- Serum-Resistant Lipoplexes in the Presence of Asialofetuin -- Anionic pH Sensitive Lipoplexes -- Liposomal siRNA Delivery -- Complexation of siRNA and pDNA with Cationic Liposomes: The Important Aspects in Lipoplex Preparation -- Effective In Vitro and In Vivo Gene Delivery by the Combination of Liposomal Bubbles (Bubble Liposomes) and Ultrasound Exposure -- Liposomal Magnetofection -- Long-Circulating, pH-Sensitive Liposomes -- Serum-Stable, Long-Circulating, pH-Sensitive PEGylated Liposomes.

With nearly one hundred years of intensive study, lipids have proven to be a vital and ever-more-promising area of cell biological research. In Liposomes: Methods and Protocols, leading experts in the related fields explore cutting-edge experimental methods involving all aspects of lipids as essential components of the cell membrane. Volume 1: Pharmaceutical Nanocarriers focuses on the state-of-the-art aspects of developing liposome-based nanoscale drug delivery techniques, delving into the depths of the emerging field of pharmaceutical and biomedical nanotechnology. As a volume in the highly successful Methods in Molecular Biology™ series, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Liposomes: Methods and Protocols promises to be an essential source of practical know-how for every investigator, young and seasoned alike, whose research area involves in one way or another phospholipids, glycolipids, or cholesterol.