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Baculovirus Expression Protocols / by Christopher D. Richardson.

Por: Richardson, Christopher D, autor.
Series (Methods in Molecular Biology, 1940-6029; 39).Editor: Totowa, NJ : Humana Press, 1995Edición: 1st edition 1995.Descripción: 1 recurso en línea (XIV, 418 páginas) : .ISBN: 9781592595266.Recursos en línea: (usuarios Universidad Europea de Valencia)Digital Resources
Contenidos:
to the Molecular Biology of Baculoviruses -- Baculovirus Transfer Vectors Miguel LÓpez- Ferber -- Insect Cell- Culture Techniques in Serum- Containing Medium -- Insect Cell Culture in Serum- Free Media -- Transfection Techniques for Producing Recombinant Baculoviruses -- Selection of Recombinant Baculoviruses by Visual Screening -- Production of recombinant baculoviruses using linearized viral DNA -- Rapid procedures for the isolation and PCR analysis of recombinant baculovirus -- Production of recombinant baculoviruses using rapid screening vectors that contain the gene for ß -Galactosidase -- Fast isolation of recombinant baculovirus by antibody screening -- Continuous foreign gene expression in transformed lepidopteran insect cells -- Scale-Up of recombinant virus and protein production in Stirred-Tank reactors -- Expression of Tissue Inhibitor of Metalloproteinases by Recombinant Baculovirus-Infected Insect Cells Cultured in an Airlift Fermentor -- Expression of Foreign Genes in Bombyx mori Larvae Using Baculovirus Vectors -- Expression of Foreign Proteins in Trichoplusia ni Larvae -- Use of Baculoviruses as Biological Insecticides -- Molecular Approaches to AIDS Vaccine Development Using Baculovirus Expression Vectors -- Expression of the Hemagglutinin Protein of Influenza Virus -- Purification of Recombinant Protein Derived fkom the Baculovirus Expression System Using Glutathione Affinity Agarose -- Purification of the Extracellular Domain of the Epidermal Growth Factor Receptor Produced by Recombinant Baculovirus-Infected Insect Cells in a 10-L Reactor -- Expression and Secretion of a Soluble Form of Myelin-Associated Glycoprotein (MAG) -- Comparison of Different Cell Lines for the Production of Recombinant Baculovirus Proteins -- Development of Lepidopteran Cell Lines.
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to the Molecular Biology of Baculoviruses -- Baculovirus Transfer Vectors Miguel LÓpez- Ferber -- Insect Cell- Culture Techniques in Serum- Containing Medium -- Insect Cell Culture in Serum- Free Media -- Transfection Techniques for Producing Recombinant Baculoviruses -- Selection of Recombinant Baculoviruses by Visual Screening -- Production of recombinant baculoviruses using linearized viral DNA -- Rapid procedures for the isolation and PCR analysis of recombinant baculovirus -- Production of recombinant baculoviruses using rapid screening vectors that contain the gene for ß -Galactosidase -- Fast isolation of recombinant baculovirus by antibody screening -- Continuous foreign gene expression in transformed lepidopteran insect cells -- Scale-Up of recombinant virus and protein production in Stirred-Tank reactors -- Expression of Tissue Inhibitor of Metalloproteinases by Recombinant Baculovirus-Infected Insect Cells Cultured in an Airlift Fermentor -- Expression of Foreign Genes in Bombyx mori Larvae Using Baculovirus Vectors -- Expression of Foreign Proteins in Trichoplusia ni Larvae -- Use of Baculoviruses as Biological Insecticides -- Molecular Approaches to AIDS Vaccine Development Using Baculovirus Expression Vectors -- Expression of the Hemagglutinin Protein of Influenza Virus -- Purification of Recombinant Protein Derived fkom the Baculovirus Expression System Using Glutathione Affinity Agarose -- Purification of the Extracellular Domain of the Epidermal Growth Factor Receptor Produced by Recombinant Baculovirus-Infected Insect Cells in a 10-L Reactor -- Expression and Secretion of a Soluble Form of Myelin-Associated Glycoprotein (MAG) -- Comparison of Different Cell Lines for the Production of Recombinant Baculovirus Proteins -- Development of Lepidopteran Cell Lines.

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