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Adhesion Protein Protocols / edited by Amanda S. Coutts.

Colaborador(es): Coutts, Amanda S, editor literario.
Series (Methods in Molecular Biology, 1940-6029; 370).Editor: Totowa, NJ : Humana Press, 2007Edición: 2nd edition 2007.Descripción: 1 recurso en línea (X, 234 páginas) : 54 ilustraciones, 2 ilustraciones a color.ISBN: 9781597453530.Recursos en línea: (usuarios Universidad Europea de Valencia)Digital Resources
Contenidos:
Manipulation of Cell-Cell Adhesion Using Bowtie-Shaped Microwells -- Separation of Cell-Cell Adhesion Complexes by Differential Centrifugation -- Analysis of Neutrophil Chemotaxis -- Analysis of Leukocyte Migration Through Monolayers of Cultured Endothelial Cells -- Biochemical Purification of Pseudopodia from Migratory Cells -- Dynamic Assessment of Cell-Matrix Mechanical Interactions in Three-Dimensional Culture -- Quantitative Analyses of Cell Adhesion Strength -- Using RNA Interference to Knock Down the Adhesion Protein TES -- Assaying Calpain Activity -- Analysis of Focal Adhesions and Cytoskeleton by Custom Microarray -- Proteomic Analysis of Cell Surface Membrane Proteins in Leukemic Cells -- Bioinformatic Analysis of Adhesion Proteins -- Analysis of Integrin Dynamics by Fluorescence Recovery After Photobleaching -- Double-Hydrogel Substrate as a Model System for Three-Dimensional Cell Culture -- In Vitro Actin Assembly Assays and Purification From Acanthamoeba.
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Manipulation of Cell-Cell Adhesion Using Bowtie-Shaped Microwells -- Separation of Cell-Cell Adhesion Complexes by Differential Centrifugation -- Analysis of Neutrophil Chemotaxis -- Analysis of Leukocyte Migration Through Monolayers of Cultured Endothelial Cells -- Biochemical Purification of Pseudopodia from Migratory Cells -- Dynamic Assessment of Cell-Matrix Mechanical Interactions in Three-Dimensional Culture -- Quantitative Analyses of Cell Adhesion Strength -- Using RNA Interference to Knock Down the Adhesion Protein TES -- Assaying Calpain Activity -- Analysis of Focal Adhesions and Cytoskeleton by Custom Microarray -- Proteomic Analysis of Cell Surface Membrane Proteins in Leukemic Cells -- Bioinformatic Analysis of Adhesion Proteins -- Analysis of Integrin Dynamics by Fluorescence Recovery After Photobleaching -- Double-Hydrogel Substrate as a Model System for Three-Dimensional Cell Culture -- In Vitro Actin Assembly Assays and Purification From Acanthamoeba.

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