The Identification of Subcellular Fractions from Mammalian Cells -- The Isolation of Nuclei and Nuclear Membranes from Rat Liver -- Isolation of Mitochondria, Mitochondrial Membranes, Lysosomes, Peroxisomes, and Golgi Membranes from Rat Liver -- Continuous-Flow Electrophoresis -- Fractionation of a Microsomal Fraction from Rat Liver or Cultured Cells -- Isolation of Plasma Membrane Sheets and Plasma Membrane Domains from Rat Liver -- The Analysis of Receptor-Mediated Endocytosis by Centrifugation in Isoosmotic Gradients -- The Isolation of Clathrin-Coated Vesicles and Purification of Their Protein Components -- Isolation of Membranes from Tissue Culture Cells -- The Isolation of Membranes from Bacteria -- Isolation and Purification of Functionally Intact Chloroplasts from Leaf Tissue and Leaf Tissue Protoplasts -- Isolation and Purification of Functionally Intact Mitochondria from Plant Cells -- Isolation of Cholinergic-Specific Synaptosomes by Immunoadsorption -- Separation and Analysis of Phospholipids by Thin Layer Chromatography -- Analysis of Phospholipids by High Performance Liquid Chromatography -- Measurement of Cholesterol in Membranes -- Analysis of Fatty Acids by Gas Liquid Chromatography -- Chemical Assays for Proteins -- Separation and Analysis of Membrane Proteins by SDS-Polyacrylamide Gel Electrophoresis -- Analysis of Membrane Proteins by Western Blotting and Enhanced Chemiluminescence -- Two-Dimensional Polyacrylamide Gel Electrophoresis of Membrane Proteins -- Isolation of a Membrane-Bound Enzyme, 5?-Nucleotidase -- The Isolation of Membrane Proteoglycans -- Oligosaccharide Mapping and Sequence Analysis of Glycosaminoglycans -- Analysis of Sugar Sequences in Glycoproteins by Glycosidase Digestion and Gel Filtration -- The Extraction and Analysis of Glycosphingolipids.