Fast Masking Of Repeated Primer Binding Sites In Eukaryotic Genomes -- Primer Design for PCR Reactions in Forensic Biology -- Design of Primers and Probes For Quantitative Real-Time PCR Methods -- Large-scale Nucleotide Sequence Alignment And Sequence Variability Assessment To Identify the Evolutionarily Highly Conserved Regions For Universal Screening PCR Assay Design: An Example Of Influenza A Virus -- Low-concentration Initiator Primers Improve The Amplification Of Gene Targets With High Sequence Variability -- Multiplex PCR Primer Design for Simultaneous Detection of Multiple Pathogens -- Degenerate Primer Design for Highly Variable Genomes -- Allele-specific Real Time Polymerase Chain Reaction As A Tool For Urate Transporter 1 Mutation Detection -- MultiPLX: Automatic Grouping And Evaluation Of PCR Primers -- In silico PCR Primer Designing and Validation -- Primer Design using Primer Express® for SYBR Green Based Quantitative PCR -- Designing Primers for SNaPshot Technique -- Rapid and Simple Method of qPCR Primer Design -- PRIMEGENSw3: A Web-based Tool for High-throughput Primer and Probe Design -- Selecting Specific PCR Primers with MFEprimer.

This volume provides an overview on design PCR primers for successful DNA amplification. Chapters focus on primer design strategies for quantitative PCR, in silico PCR primer design , and primer design using software. Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, PCR Primer Design, Second Edition seeks to aid molecular biology students, researchers, professors and PCR enthusiasts.