A Blueprint for DNA Vaccines Design -- Enhancement of Plasmid-Mediated Transgene Expression -- Strategies for Improving DNA Vaccines Performance -- Enhancement of DNA Vaccine Efficacy by Intracellular Targeting Strategies -- Progresses in DNA-Based Heterologous Prime-Boost Immunization Strategies -- Development of Antibiotic-Free Selection System for Safer DNA Vaccination -- Electroporation-Based DNA Delivery Technology: Methods for Gene Electrotransfer to Skin -- DNA Vaccination in Skin Enhanced by Electroporation -- Intradermal Vaccination by DNA Tattooing -- Microneedle Applications for DNA Vaccine Delivery to the Skin -- Multivalent DNA-Based Vectors for DNA Vaccines Delivery -- Superparamagnetic Nanoparticles Delivery of DNA Vaccine -- Plasmid Fermentation Process for DNA Immunization Applications -- Pharmaceutical Grade Large Scale Plasmid DNA Manufacturing Process -- Protective and Therapeutic DNA Vaccination Against Allergic Diseases -- Immunotherapy for Alzheimer's Disease: DNA- and Protein-Based Epitope Vaccines -- Tetravalent DNA Vaccine Product as a Vaccine Candidate Against Dengue -- DNA Vaccination as a Treatment for Chronic Kidney Disease.

DNA Vaccines: Methods and Protocols, Third Edition explores innovative approaches and technologies used to design, deliver, and enhance the efficacy of DNA vaccines.  Featuring applications which should be of great value in moving vaccines from research to clinic, this detailed volume includes sections on DNA vaccine design and enhancement, delivery systems, production, purification, and quality, as well as chapters on new vaccine applications.  Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, DNA Vaccines: Methods and Protocols, Third Edition serves the important role of further documenting the potential of the DNA vaccination as a platform technology for treatment and prevention of human disease.