Analysis of Frequency and Phenotype of Antigen-Specific T Cells -- B Cell Helper Assays -- transkingdom RNA Interference (tkRNAi): A Novel Method to Induce Therapeutic Gene Silencing -- Flow Cytometry and Cell Activation -- Investigating T Cells by Polychromatic Flow Cytometry -- Generation of Human T Cell Clones -- Limiting Dilution Analysis of Antigen-Specific T Cells -- T Cell Epitope-Mapping by Cytokine Gene Expression Assay -- Cytokine Multiplex Immunoassay: Methodology and (Clinical) Applications -- Purification of the T Cell Antigen Receptor and Analysis by Blue-Native PAGE -- Non-Replicating Recombinant Vaccinia Virus Expressing CD80 to Enhance T-Cell Stimulation.

With a wide variety of investigative approaches, T cell immunology is a vital and open field of study. In T Cell Protocols, Second Edition, an international panel of experts contribute fully updated classic protocols as well as newly established novel techniques for the study of T lymphocyte biology. Written in the highly successful Methods in Molecular Biology™ series format, the chapters in this volume provide brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections which collect expert tips on troubleshooting and avoiding known pitfalls. Up-to-date and easy to use, T Cell Protocols, Second Edition is an ideal guide for young investigators new to the complex field of immunology as well as a valuable, concise resource for experienced scientists searching for clear, efficacious descriptions of novel methods.