The Retroviral Life Cycle and the Molecular Construction of Retrovirus Vectors -- Construction of Retro viral Packaging Cell Lines -- Making High Titer Retroviral Producer Cells -- Methods of Titrating Nonselectable Recombinant Retroviruses -- Selectable Markers for Eukaryotic Cells -- Virus Genome Detection by the Polymerase Chain Reaction (PCR) -- Syncytial Assays -- Neutralization of Virus -- Vesicular Stomatitis Virus Pseudotypes of Retroviruses -- Cell Lineage Studies Using Retroviruses -- Retroviral Vectors as Insertional Mutagens -- Assessment of Compounds for Anti-HIV Activity -- Analysis of Protein Factors that Interact with Adenovirus Early Promoters -- COS Cell Expression -- Papillomaviruses and Assays for Transforming Genes -- Herpes Simplex Virus Life Cycle and the Design of Viral Vectors -- Growth of Herpes Simplex Virus and Purification of Viral DNA -- Vectors Based on Epstein-Barr Virus -- Immortalization of Human B-Lymphocytes by Epstein-Barr Virus -- Vaccinia Virus as an Expression Vector -- Isolation and Handling of Recombinant Vaccinia Viruses -- Design and Construction of Poliovirus Epitope Expression Vectors -- Growth and Characterization of Poliovirus Antigen Chimeras -- Expression Vectors for the Construction of Hybrid Ty-VLPs -- Production and Purification of Hybrid Ty-VLPs -- Baculovirus Expression Vectors -- Baculovirus Expression Vectors -- Baculovirus Expression Vectors.