Using Fluorescent Proteins to Study Poxvirus Morphogenesis -- Molecular Imaging of Human Embryonic Stem Cells -- Use of a GFP-PML-Expressing Cell Line as a Biosensor for Human Cytomegalovirus Infection -- Dual-Color Imaging of Tumor Angiogenesis -- Use of GFP to Analyze Morphology, Connectivity, and Function of Cells in the Central Nervous System -- Expression of EGFP by Adenovirus-Mediated Gene Transfer in the Central Nervous System -- Adenovirus-Mediated Delivery of Short Hairpin RNA (shRNA) Mediates Efficient Gene Silencing in Terminally Differentiated Cardiac Myocytes -- Microscopic Analysis of Adenoviral Decontamination Using GFP Adenovirus with Comparable Sensitivity to Flow Cytometry -- Production of Multicistronic HIV-1 Based Lentiviral Vectors -- Using an EGFPmeter to Evaluate the Lentiviral Vector Production: Tricks and Traps -- Use of a Macrophage-Tropic GFP-Tagged Human Immunodeficiency Virus Type 1 (HIV-1) to Study Viral Reservoirs -- GFP-Lentiviral Vectors Targeting for NeuroAIDS -- Construction of Cell-Type Specific Promoter Lentiviruses for Optically Guiding Electrophysiological Recordings and for Targeted Gene Delivery -- Selective Protein Expression Within the CNS Using Hybrid Lentivirus -- Construction and Analysis of Alphaherpesviruses Expressing Green Fluorescent Protein -- The Use of Green Fluorescent Fusion Proteins to Monitor Herpes Simplex Virus Replication -- Modified Bovine Herpesvirus 1 for Protein Secretion -- Identification of Viral Peptide Fragments for Vaccine Development -- Use of GFP to Investigate Expression of Plant-Derived Vaccines -- Studies of the Role and Function of Barley Stripe Mosaic Virus Encoded Proteins in Replication and Movement Using GFP Fusions -- Sendai Virus for Cancer Immunotherapy -- Poxvirus Tropism for Primary Human Leukocytes and Hematopoietic Cells -- Reverse Genetics of Nipah Virus to Probe Viral Pathogenicity -- Baculovirus-Mediated Gene Transfer into Mesenchymal Stem Cells.

Over the last ten years, Green Fluorescent Proteins, along with the other spectral variants, have emerged from near obscurity to become a powerful and versatile tool in scientific research. In Viral Applications of Green Fluorescent Protein: Methods and Protocols, leading investigators from around the world contribute detailed examples of both the construction and application of fluorescent proteins delivered by viruses in a format crafted to produce rapid, readily reproducible results. Written in the style of the popular and successful Methods in Molecular Biology™ series, the chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls. Cutting-edge and easy to use, Viral Applications of Green Fluorescent Protein: Methods and Protocols supplies researchers with an ideal guide to the many uses of GFP and a vital starting point for future studies utilizing this highly adaptable protein.