Small Non-Coding RNAs: A Quick Look in the Rear-View Mirror -- Alcoholic Precipitation of Small Non-Coding RNAs -- Quantification and Quality Control of a Small Non-Coding RNA Preparation -- Impact of RNA Isolation Protocols on RNA Detection by Northern Blotting -- Improved Northern Blot Detection of Small RNAs Using EDC Crosslinking and DNA/LNA Probes -- Direct Cloning of Double Stranded RNAs -- Detection and Labeling of Small Non-Coding RNAs by Splinted Ligation -- Fluorescence in situ Hybridization of Small Non-Coding RNAs -- RT-qPCR Based Quantification of Small Non-Coding RNAs -- Stem-Loop RT-PCR Based Quantification of Small Non-Coding RNAs -- miR-RACE: An Effective Approach to Accurately Determine the Sequence of Computationally Identified miRNAs -- Probing Small Non-Coding RNAs Structures -- cDNA Library Generation for the Analysis of Small RNAs by High Throughput Sequencing -- CLIP-seq to Discover Transcriptome-Wide Imprinting of RNA Binding Proteins in Living Cells -- Microarray Analysis of Small Non-Coding RNAs -- RLM-RACE, PPM-RACE and qRT-PCR, an Integrated Strategy to Accurately Validate miRNA Target Genes -- Dual Luciferase Gene Reporter Assays to Study miRNA Function -- Gene Expression Knockdown by Transfection of siRNAs into Mammalian Cells -- Efficient and Selective Knockdown of Small Non-Coding RNAs -- Cell-SELEX: In Vitro Selection of Synthetic Small Specific Ligands -- Small Non-Coding RNAs and Aptamers in Diagnostics and Therapeutics.

This volume contains state-of-the-art methods tackling all aspects of small non-coding RNAs biology.  Small Non-Coding RNAs: Methods and Protocols guides readers through customized dedicated protocols and technologies that will be of valuable help to all those willing to contribute deciphering the numerous functions of small non-coding RNAs. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubles troubleshooting and avoiding known pitfalls. Instructive and practical, Small Non-Coding RNAs: Methods and Protocols reaches out to biochemists, cellular, and molecular biologists already working in the field of RNA biology  and to those just starting to study small non-coding RNAs.