Experimental Models of Wound Healing -- Excisional Wound Healing -- Methods in Reepithelialization -- Incisional Wound Healing -- Animal Models of Ischemic Wound Healing -- Corneal Injury -- Subcutaneous Sponge Models -- A Mouse Model of Burn Wounding and Sepsis -- A Porcine Burn Model -- Wound Healing in Airways In Vivo -- Murine Models of Intestinal Anastomoses -- Murine Model of Peritoneal Adhesion Formation -- Methods for Investigating Fetal Tissue Repair -- Growth of Human Blood Vessels in Severe Combined Immunodeficient Mice -- Tissue Repair in Models of Diabetes Mellitus -- Wound Healing Studies in Transgenic and Knockout Mice -- Wound Repair in Aging -- Specimen Collection and Analysis -- Suction Blister Model of Wound Healing -- Implantable Wound Healing Models and the Determination of Subcutaneous Collagen Deposition in Expanded Polytetrafluoroethylene Implants -- The Fibroblast-Populated Collagen Lattice -- A Quantifiable In Vitro Model to Assess Effects of PAI-1 Gene Targeting on Epithelial Cell Motility -- Human Skin Organ Culture -- In Vitro Matrigel Angiogenesis Model -- Analysis and Manipulation of Wound Healing -- Quantification of Wound Angiogenesis -- In Vivo Matrigel Migration and Angiogenesis Assays -- Endothelial Cell Migration Assay -- Analysis of Collagen Synthesis -- Method for Detection and Quantitation of Leukocytes During Wound Healing -- Detection of Reactive Oxygen Intermediate Production by Macrophages -- Measurement of Chemokines at the Protein Level in Tissue -- Methods of Measuring Oxygen in Wounds -- Isolation, Culture, and Characterization of Human Intestinal Smooth Muscle Cells -- Use of High-Throughput Microarray Membranes for cDNA Analysis of Cutaneous Wound Repair -- Particle-Mediated Gene Therapy of Wounds.

During the past decade the significant progress made in cell and molecular biotechnology has led to exciting discoveries in the field of tissue repair, producing a wealth of information about the healing process. In Wound Healing: Methods and Protocols, expert researchers and physicians not only describe classic and contemporary laboratory methods for studying tissue repair using a broad range of wound healing models, but also provide sensitive assays for the assessment of tissue healing. The systems detailed include human, animal, in vitro, in vivo, and impaired healing models, as well as models in which underlying systemic and genetic conditions influence the healing process. Useful for the study of many biological processes, including angiogenesis, epidermal differentiation and repair, acute inflammation, ECM synthesis, and remodeling, these powerful models illustrate biochemical, molecular, and surgical techniques designed for the analysis and manipulation of the healing wound, and provide step-by-step instructions to ensure successful results. In many cases, several different approaches to a single process are examined, with sample results and analyses given to help users in selecting the approach most suited to the problem at hand. Comprehensive and enlightening, Wound Healing: Methods and Protocols offers both basic and clinical scientists a thought-provoking and highly practical collection of widely used model systems and methods for studying the injury repair process.