Expression and Purification of Recombinant Proteins in Escherichia Coli with a His6 or Dual His6-MBP Tag -- Protein Crystallization -- Advanced Methods of Protein Crystallization -- The 'Sticky Patch' Model of Crystallization and Modification of Proteins for Enhanced Crystallizability -- Crystallization of Membrane Proteins: and Overview -- Locating and Visualizing Crystals for X-ray Diffraction Experiments -- Collection of X-ray Diffraction Data from Macromolecular Crystals -- Identifying and Overcoming Crystal Pathologies: Disorder and Twinning -- Applications of X-ray Micro-beam for Data Collection -- Serial Synchrotron X-ray Crystallography (SSX) -- Time-Resolved Macromolecular Crystallography at Modern X-ray Sources -- Structure Determination Using X-ray Free-electron Laser Pulses -- Processing of XFEL Data -- Many Ways to Derivatize Macromolecules and their Crystals for Phasing -- Experimental Phasing: Substructure Solution and Density Modification as Implemented in SHELX -- Contemporary Use of Anomalous Diffraction in Biomolecular Structure Analysis -- Long-Wavelength X-ray Diffraction and its Applications in Macromolecular Crystallography -- Acknowledging Errors: Advanced Molecular Replacement with Phaser -- Rosetta Structure Prediction as a Tool for Solving Difficult Molecular Replacement Problems -- Radiation Damage in Macromolecular Crystallography -- Boxes of Model Building and Visualization -- Structure Refinement at Atomic Resolution -- Low Resolution Refinement of Atomic Models against Crystallographic Data -- Stereochemistry and Validation of Macromolecular Structures -- Validation of Protein-Ligand Crystal Structure Models: Small Molecule and Peptide Ligands -- Protein Data Bank - the Single Global Macromolecular Structure Archive Managed by the Worldwide Protein Data Bank -- Databases, Repositories and Other Data Resources in Structural Biology.

This volume provides methods for modern macromolecular crystallography, including all steps leading to crystal structure determination and analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protein Crystallography aims to ensure successful results in the further study of this vital field.