DNase I Footprinting -- Quantitative DNA Footprinting -- Uranyl Photoprobing of DNA Structures and Drug-DNA Complexes -- Diethylpyrocarbonate and Osmium Tetroxide as Probes for Drug-Induced Changes in DNA Conformation In Vitro -- Footprinting Studies with Nucleosome-Bound DNA -- A Gel Mobility Shift Assay for Probing the Effect of Drug-DNA Adducts on DNA-Binding Proteins -- An Oligonucleotide Crosslinking Assay for the Analysis of Individual Drug-Binding Sites -- DNA Polymerase Inhibition Assay (PIA) for the Detection of Drug-DNA Interactions -- Transcriptional Footprinting of Drug-DNA Interactions -- Determination of the DNA Sequence Specificity of Alkylation Damage Using Cleavage-Based Assays -- PCR-Based Methods for Detecting DNA Damage and Its Repair at the Subgene and Single Nucleotide Levels in Cells -- Analysis of DNA-Binding Antibodies -- Immunofluorescent Staining of Chromosomes with DNA-Binding Antibodies -- Optical Absorbance and Fluorescence Techniques for Measuring DNA-Drug Interactions -- Evaluation of Drug-Nucleic Acid Interactions by Thermal Melting Curves -- Electric Dichroism -- Calorimetric Techniques for Studying Drug-DNA Interactions -- Methods for the Studies of Drug Dissociation from DNA.

In Drug-DNA Interactions, expert researchers describe powerful molecular techniques designed to illuminate and explore the interaction of drugs and ligands with DNA, often perfected in their own laboratories, . These easily reproducible methods involve sequence recognition properties, as well as physical approaches to measuring both the strength of interaction and the mode of drug binding to DNA. Drug interactions are also examined from a cellular perspective and for their usefulness in the development of new therapeutic agents. Drug-DNA Interactions will be highly useful to all investigators needing the kind of detailed experimental and technical information often omitted from scientific papers. Those wishing to expand their repertoire of experimental techniques or to improve their current techniques will also find these powerful methods particularly helpful in elucidating the action of existing therapeutic agents, as well as in designing new anticancer drugs with improved action and reduced toxicity.