B-cell Epitope Mapping -- What Is a B-Cell Epitope? -- Whole Antigen Methods -- Structural Basis of Antibody-Antigen Interactions -- Epitope Mapping of Antibody-Antigen Complexes by Nuclear Magnetic Resonance Spectroscopy -- A Solid-Phase Mutual Inhibition Assay with Labeled Antigen -- Epitope Mapping by Surface Plasmon Resonance -- Proteolytic Fragmentation for Epitope Mapping -- Epitope Mapping by Proteolysis of Antigen-Antibody Complexes -- Identifying Residues in Antigenic Determinants by Chemical Modification -- Epitope Mapping by Differential Chemical Modification of Antigens -- Peptide Library Approaches -- Linear B-Cell Epitope Mapping Using Enzyme-Linked Immunosorbent Assay for Libraries of Overlapping Synthetic Peptides -- Antibody Epitope Mapping Using SPOT™ Peptide Arrays -- Peptide Microarrays for Profiling of Modification State-Specific Antibodies -- Epitope Mapping Using Phage Display Peptide Libraries -- Antibody Epitope Mapping Using De Novo Generated Synthetic Peptide Libraries -- Antibody Specificity Profiling on Functional Protein Microarrays -- Peptide Microarrays for Determination of Cross-Reactivity -- Profiling of Antibody Signatures in Biological Fluids. -- Epitope Mapping Using Randomly Generated Peptide Libraries -- Probing the Epitope Signatures of IgG Antibodies in Human Serum from Patients with Autoimmune Disease -- Microarrayed Allergen Molecules for Diagnostics of Allergy -- Monitoring B Cell Response to Immunoselected Phage-Displayed Peptides by Microarrays -- Antigen Expressed from Recombinant DNA -- Epitope Mapping Using Homolog-Scanning Mutagenesis -- Epitope Mapping by Region-Specified PCR-Mutagenesis -- Epitope Mapping Using Phage-Display Random Fragment Libraries -- B-Cell Epitope Prediction -- Prediction of Linear B-cell Epitopes -- T-Cell Epitope Mapping -- Molecular Recognition of Diverse Ligands by T-Cell Receptors -- Identification of Human MHC Class I Binding Peptides using the iTOPIA™? Epitope Discovery System -- T-Cell Epitope Mapping in Mycobacterium tuberculosis Using PepMixes Created by Micro-Scale SPOT™? Synthesis -- High-Throughput T-Cell Epitope Discovery Through MHC Peptide Exchange -- T-Cell Epitope Processing (The Epitope Flanking Regions Matter) -- Identification of MHC Class II Binding Peptides: Microarray and Soluble MHC Class II Molecules -- T-Cell Epitope Mapping -- Identification and Validation of T-Cell Epitopes Using the IFN-? EliSpot Assay.

Given the versatile utility of the determinination of epitopes, beneficial to a wide variety of scientists from immunologists to structural biologists to biotechnologists, the need for a thorough, state-of-the-art collection of experimental protocols is clear. In Epitope Mapping Protocols, Second Edition, expert contributors from a broad spectrum of scientific backgrounds update and expand the successful first edition with cutting-edge techniques and applications, including approaches to both antibody or B-cell epitope mapping and T-cell epitope mapping as well as a new section on the profiling of antibody signatures in biological fluids. Written in the popular Methods in Molecular Biology™ series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Epitope Mapping Protocols, Second Edition is a reliable and valuable reference for all those who wish to understand and further investigate the diversifying field of epitope mapping.