The Animal Cap Assay -- Cell and Tissue Transplantation in Zebrafish Embryos -- Ribonuclease Protection Analysis of Gene Expression in Xenopus -- Quantitative Analysis of mRNA Levels in Xenopus Embryos by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) -- Wholemount In Situ Hybridization of Xenopus and Zebrafish Embryos -- In Situ Hybridization to Sections of Xenopus Embryos -- Zebrafish Immunohistochemistry -- Immunohistochemistry of Xenopus Embryos -- Preparation and Testing of Synthetic mRNA for Microinjection -- Microinjection into Xenopus Oocytes and Embryos -- Microinjection into Zebrafish Embryos -- Expression from DNA Injected into Xenopus Embryos -- Promoter Analysis in Zebrafish Embryos -- Transient Transgenesis in Xenopus laevis Facilitated by AAV-ITRs -- Band-Shift Analysis Using Crude Oocyte and Embryo Extracts from Xenopus laevis -- DNA-Footprinting using Crude Embryonic Extracts from Xenopus laevis -- Mapping Protein-DNA Interactions Using In Vivo Footprinting.

In Molecular Methods in Developmental Biology: Xenopus and Zebrafish, Matthew Guille assembles a hands-on collection of basic and essential molecular and embryological techniques for studying Xenopus and zebrafish. Easily reproducible and designed to succeed, these detailed methods include cellular techniques, techniques for the quantitative and spatial analysis of mRNA and proteins, and techniques for the expression of gene products in embryos. More specialized methods enable users to analyze promoters and transcription factors during early development, and include gel shift assays, as well as in vitro and in vivo footprinting. Wherever possible, these experimental approaches are applied to both Xenopus and zebrafish. Molecular Methods in Developmental Biology: Xenopus and Zebrafish affords newcomers rapid access to a wide variety of key techniques in developmental research, and offers experienced investigators both new techniques from experts who have fine-tuned them for best results, and a plethora of time-saving tips. State-of-the-art and readily reproducible, these powerful methods constitute today's gold-standard laboratory manual for understanding the interactions responsible for development.