General Preparation of Material and Staining of Sections -- Negative Staining of Thinly Spread Biological Particulates -- Preparation of Thin-Film Frozen-Hydrated/ Vitrified Biological Specimens for Cryoelectron Microscopy -- The Production of Cryosections Through Fixed and Cryoprotected Biological Material and Their Use in Immunocytochemistry -- High-Pressure Freezing for Preservation of High Resolution Fine Structure and Antigenicity for Immunolabeling -- The Application of LR Gold Resin for Immunogold Labeling -- Low-Temperature Embedding in Acrylic Resins -- Quantitative Aspects of Immunogold Labeling in Embedded and Nonembedded Sections -- Microwave Processing Techniques for Electron Microscopy: A Four-Hour Protocol -- Electron Microscopic Enzyme Cytochemistry -- In Situ Molecular Hybridization Techniques for Ultrathin Sections -- Preparation of the Fission Yeast Schizosaccharomyces pombe for Ultrastructural and Immunocytochemical Study -- Preparation of Double/Single-Stranded DNA and RNA Molecules for Electron Microscopy -- Applications of Electron Microscopy for Studying Protein-DNA Complexes -- X-Ray Microanalysis Techniques.

In Electron Microscopy Methods and Protocols, well-practiced experts describe in detail the key electron microscopy techniques used for examining cells, tissue, biological macromolecules, molecular structure, and their interactions. With emphasis on cryotechniques for quantitative biological X-ray microanalysis, the book also includes those methods that use antibodies to locate proteins within cells and that prepare and analyze nucleic acids, proteins, and protein-nucleic acid complexes. Numerous immunogold labeling techniques for precise ultrastructural localization, distribution, and quantitation of macromolecules in cryo-fixed or chemically-fixed cells are described in sufficient detail to provide practical insight into their advantages and limitations. Electron Microscopy Methods and Protocols offers both newcomers and established researchers wanting to expand their repertoire of cutting-edge electron microscopy techniques-each optimized for reproducibility and robust results-today's gold-standard laboratory manual.