NO-cGMP: The Past, the Present and the Future -- cGMP Dependent Protein Kinases (cGK) -- Enzyme Assays for cGMP Hydrolysing Phosphodiesterases -- Radioimmunoassay for the Quantification of cGMP Levels in Cells and Tissues -- Hyperspectral Imaging of FRET-based cGMP Probes -- Visualization of cGMP with cGi Biosensors -- Advances and techniques to measure cGMP in intact cardiomyocytes -- Real-time Monitoring the Spatio-temporal Dynamics of Intracellular cGMP in Vascular Smooth Muscle Cells -- Methods for Identification of cGKI Substrates -- Approaches for Monitoring PKG1a Oxidative Activation -- Analysis of cGMP Signaling in Adipocytes -- A Genetic Strategy for the Analysis of Individual Axon Morphologies in cGMP Signalling Mutant Mice -- Receptor Binding Assay for NO-Independent Activators of soluble Guanylate Cyclase -- Direct Intrathecal Drug Delivery in Mice for Detecting In Vivo Effects of cGMP on Pain Processing -- The Geisler Method: Tracing Activity Dependent cGMP Plasticity Changes Upon Double Detection of mRNA and Protein on Brain Slices -- Detection of cGMP in the Degenerating Retina.

Recently, many ground-breaking steps have been made towards  better understanding NO/cGMP/PKG pathways, its components, substrates, and their localization within a given cell. These advances were only possible due to the development of sophisticated new techniques in the field. In Guanylate Cyclase and Cyclic GMP: Methods and Protocols expert researchers in the field seek to provide an overview of novel techniques to identify various elements of the NO/cGMP/PKG pathway and further characterize their function, signaling, localization, and importance on a cellular level and in whole animal models providing a higher patho-/physiological integration and relevance.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.       Authoritative and practical, Guanylate Cyclase and Cyclic GMP: Methods and Protocols seeks to provide  scientist current methods and a useful guide towards the possibility to apply these techniques to their own research.