Introduction: Medical Significance of H. pylori -- Detection of H. pylori Infection by Biopsy Urease, Histology, and Culture -- Serology and Urea Breath Test in the Diagnosis of H. pylori Infection -- The Detection of H. pylori by the Polymerase Chain Reaction -- PCR for the Detection of H. pylori in Gastric Juice Aspirates and Environmental Water Samples -- In vitro Susceptibility Testing of H. pylori -- Defined Media for H. pylori -- Studies of Respiration in H. pylori -- Use of NMR to Study H. pylori Metabolism -- Isolation of H. pylori Genomic DNA and Restriction Analysis -- Ribosomal RNA Gene Restriction Pattern Analysis (Ribotyping) of H. pylori -- Differentiation of H. pylori Strains Using PCR RFLP -- PCR-DNA Sequence Typing -- H. pylori DNA Fingerprinting Using the Arbitrarily Primed PCR (AP-PCR) or Random Amplified Polymorphic DNA (RAPD) Method -- Molecular Methods for Detecting Ulcerogenic Strains of H. pylori -- H. pylori DNA Transformation by Natural Competence and Electroporation -- Random Mutagenesis of the H. pylori Genome -- Construction of a Genomic Map of H. pylori by Pulsed-Field Gel Electrophoresis (PFGE) -- Molecular Characterization of H. pylori Surface Antigens -- Isolation and Characterization of Porins from H. pylori -- Methods for the Identification of H. pylori Host Receptors -- Host Responses to H. pylori -- Nonprimate Animal Models of H. pylori Infection -- The Nonhuman Primate Model for H. pylori Infection.

In Helicobacter pylori Protocols, leading experts bring together an outstanding collection state-of-the-art protocols for the identification and molecular manipulation of H. pylori. The authoritative contributors supply detailed and readily reproducible protocols for culturing H. pylori, for the isolation and restriction endonuclease digestion of H. pylori chromosomal DNA, and for the transformation and insertional mutagenesis of H. pylori. They also provide molecular epidemiological techniques, including ribotyping, PCR-RFLP, and RAPD-PCR. These procedures have been developed to solve the difficult technical problems created by the application of today's powerful bacterial genetic and molecular cloning techniques to H. pylori. Helicobacter pylori Protocols contains a wide variety of stand-alone techniques that are unfailingly reproducible. The book is certain to become a standard experimental and clinical laboratory reference for all those engaged in H. pylori work today.