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Antibody Engineering Protocols / edited by Sudhir Paul.

Colaborador(es): Paul, Sudhir, editor literario.
Series (Methods in Molecular Biology, 1940-6029; 51).Editor: Totowa, NJ : Humana Press, 1995Edición: 1st edition 1995.Descripción: 1 recurso en línea (XII, 450 páginas) : .ISBN: 9781592595389.Recursos en línea: (usuarios Universidad Europea de Valencia)Digital Resources
Contenidos:
SEQHUNT -- Molecular Modeling of Antibody-Combining Sites -- Structure and Properties of Human Immunoglobulin Light-Chain Dimers -- Crystallographic and Chromatographic Methods for Study of Antibody Light Chains and Other Proteins -- Detection of Human Variable Gene Family Expression at the Single-Cell Level -- Purification of Reduced and Alkylated Antibody Subunits -- Murine Monoclonal Antibody Development -- Comparative Properties of Polyclonal and Monoclonal Antibodies -- Evaluation of Antibody Clonality -- Affinity Immunoblotting -- Epitope and Idiotope Mapping Using Monoclonal Antibodies -- Anti-Idiotypic Antibodies That Mimic Opioids -- Catalytic Antibodies -- Preparation and Assay of Acetylcholinesterase Antibody -- DNA Hydrolysis by Antibodies -- Screening Strategies for Catalytic Antitransition-State Analog Antibodies -- Expression of Chimeric Immunoglobulin Genes in Mammalian Cells -- Single-Chain Anti-DNA Fv -- Molecular Cloning of Antiground-State Proteolytic Antibody Fragments -- Cloning and Bacterial Expression of an Esterolytic sFv -- Site-Directed Mutagenesis of Antibody-Variable Regions -- Synthetic Antibody Gene Libraries for In Vitro Affinity Maturation -- Chaperonins in Phage Display of Antibody Fragments -- Phage-Display Libraries of Murine and Human Antibody Fab Fragments -- Selection of Human Immunoglobulin Light Chains from a Phage-Display Library -- Purification of Antibody Light Chains by Metal Affinity and Protein L Chromatography -- Rapid Purification of Recombinant Antibody Fragments for Catalysis Screening -- Assay of Radiolabeled VIP Binding and Hydrolysis by Antibodies -- Methods of Measuring Thyroglobulin and Peptide-Methylcoumarinamide Hydrolysis by Autoantibodies -- Radiolabeling of Antibodies for Therapy and Diagnosis.
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SEQHUNT -- Molecular Modeling of Antibody-Combining Sites -- Structure and Properties of Human Immunoglobulin Light-Chain Dimers -- Crystallographic and Chromatographic Methods for Study of Antibody Light Chains and Other Proteins -- Detection of Human Variable Gene Family Expression at the Single-Cell Level -- Purification of Reduced and Alkylated Antibody Subunits -- Murine Monoclonal Antibody Development -- Comparative Properties of Polyclonal and Monoclonal Antibodies -- Evaluation of Antibody Clonality -- Affinity Immunoblotting -- Epitope and Idiotope Mapping Using Monoclonal Antibodies -- Anti-Idiotypic Antibodies That Mimic Opioids -- Catalytic Antibodies -- Preparation and Assay of Acetylcholinesterase Antibody -- DNA Hydrolysis by Antibodies -- Screening Strategies for Catalytic Antitransition-State Analog Antibodies -- Expression of Chimeric Immunoglobulin Genes in Mammalian Cells -- Single-Chain Anti-DNA Fv -- Molecular Cloning of Antiground-State Proteolytic Antibody Fragments -- Cloning and Bacterial Expression of an Esterolytic sFv -- Site-Directed Mutagenesis of Antibody-Variable Regions -- Synthetic Antibody Gene Libraries for In Vitro Affinity Maturation -- Chaperonins in Phage Display of Antibody Fragments -- Phage-Display Libraries of Murine and Human Antibody Fab Fragments -- Selection of Human Immunoglobulin Light Chains from a Phage-Display Library -- Purification of Antibody Light Chains by Metal Affinity and Protein L Chromatography -- Rapid Purification of Recombinant Antibody Fragments for Catalysis Screening -- Assay of Radiolabeled VIP Binding and Hydrolysis by Antibodies -- Methods of Measuring Thyroglobulin and Peptide-Methylcoumarinamide Hydrolysis by Autoantibodies -- Radiolabeling of Antibodies for Therapy and Diagnosis.

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