Overview -- The Impact of Molecular Technology on STD Control -- Molecular Techniques for the Detection of Sexually Transmitted Pathogens -- Neisseria gonorrhoeae -- Molecular Diagnosis of Chlamydia trachomatis Infections by Probe Hybridization, PCR, LCR,TMA, and Q-? Replicase -- Haemophilus ducreyi Detection by PCR -- Detection of Treponema pallidum, Haemophilus ducreyi, and Herpes Simplex Virus by Multiplex PCR -- Detection of Genital Mycoplasmas by PCR -- Hepatitis B Virus -- Molecular Diagnosis of HIV-1 by PCR -- Research Techniques for Epidemiology and the Management of STDs -- Genotyping of Neisseria gonorrhoeae by Pulse Field Gel Electrophoresis and PCR -- Genotyping Chlamydia trachomatis by PCR -- Human Papillomavirus Detection by PCR and Typing by Dot-Blot -- Quantitation of HIV-1 RNA in Dried Plasma Spots (DPS) -- Collection and Processing of Seminal Plasma for the Quantitation of HIV-1 RNA by NASBA and RT-PCR -- Molecular Technology for STD Diagnosis and Research: the New Challenges -- Molecular Techniques for HIV and STDs.

In Sexually Transmitted Diseases: Methods and Protocols, Rosanna Peeling and P. Frederick Sparling have created a comprehensive collection of cutting-edge molecular protocols for the laboratory diagnosis and study of sexually transmitted diseases, including HIV. The methods-each crafted by a leading expert for comprehensiveness and genuine laboratory utility-cover the major sexually transmitted pathogens, including Neisseria gonorrhoeae, Chlamydia trachomatis, Haemophilius ducreyl, Treponema pallidum, and herpes simplex. Additional molecular techniques are included for detecting the genital mycoplasmas, hepatitis B virus, HIV-1, human papillomavirus. This book will significantly enhance our ability to detect sexually transmitted infections and to conduct research to further our understanding of sexually transmitted diseases. Eminently practical and up-to-date, Sexually Transmitted Disease Protocols provides a comprehensive collection of reliable molecular methods for STD diagnosis, research, and patient management.