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008			170111s2017 xxu| s |||| 0|eng d
020			_a9781493967803
024	7		_a10.1007/978-1-4939-6780-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-VaUE
050			_aQP609.C94 _b2017 EB
245	1	0	_aCD95 _bMethods and Protocols _cedited by Patrick Legembre.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XI, 230 páginas) _b63 ilustraciones, 38 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1557
505	0		_aProduction of the Non-apoptotic Metalloprotease-cleaved CD95L and its Cytotoxic Recombinant Counterpart Designed Ig-CD95L -- CD95 Stimulation with CD95L and DISC Analysis -- Immunoprecipitation of Death Inducing Signaling Complex by Caspase-8 -- In vitro Evaluation of the Apoptosis Function in Human Activated T cells -- Proximity Ligation Assay (PLA) to Evaluate DISC and MISC Composition -- Fluorometric Methods for Detection of Mitochondrial Membrane Depolarization -- Generation and Application of Bioluminescent CD95 Ligand Fusion Proteins -- CD95-mediated Calcium Signaling -- CD95-mediated Proton Regulation -- Study of the CD95-mediated Non-apoptotic Signaling Pathway - PI3K -- Organelle Separation and Cell Signaling -- Boyden Chamber Assay to Study of Cell Migration Induced by Metalloprotease Cleaved-CD95L -- Isolation of Lipid Rafts through Discontinuous Sucrose Gradient Centrifugation and Fas/CD95 Death Receptor Localization in Raft Fractions -- Quantifying CD95/cl-CD95L Implications in Cell Mechanics And Membrane Tension by Atomic Force Microscopy Based Force Measurements -- Sketching of CD95 Oligomers by In Silico Investigations -- Site-specific Detection of Tyrosine Phosphorylated CD95 Following Protein Separation by Conventional and Phospho-Protein Affinity SDS-PAGE -- Detection of S-acylated CD95 by Acyl-biotin Exchange -- Exploration of Fas S-nitrosylation by the Biotin Switch Assay -- Method to Measure Sphingomyelin Synthase Activity Changes in Response to CD95L -- Liquid Chromatography-High Resolution Mass Spectrometry Method to Study Sphingolipid Metabolism Changes in Response to CD95L -- CD95 and the MRL-lpr Mouse Model.
520			_aThis volume provides the most commonly used methods and protocols to study the apoptotic and non-apoptotic roles of CD95. Chapters explore molecular, biochemical, cellular methods and animal models to in order to better understand the biological functions of this cytokine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, CD95: Methods and Protocols aims to foster original research on CD95/CD95L couple. .
988			_aSpringer_Protocols_2017
650		7	_2embne _96440 _aBiología
700	1		_aLegembre, Patrick _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493967780
776	0	8	_iPrinted edition: _z9781493967797
776	0	8	_iPrinted edition: _z9781493982981
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6780-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
998			_db