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007			cr nn 008mamaa
008			100301s1992 xxu| s |||| 0|eng d
020			_a9781592596287
024	7		_a10.1385/0896032140 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-VaUE
050			_aQP357 _b2008 EB
245	1	0	_aPractical Cell Culture Techniques _cedited by Alan A. Boulton, Glen B. Baker, Wolfgang Walz.
264		1	_aTotowa, NJ _bHumana Press _c2008
300			_a1 recurso en línea (XIX, 392 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aNeuromethods _x1940-6045 _v23
505	0		_aBasic Techniques for Cell Culturing -- Identification of Cell Types in Neural Cultures -- Environmental Influences on Cells in Culture -- Detection and Analysis of Growth Factors Affecting Neural Cells -- The Role of Cell Adhesion Molecules in Neurite Growth -- Three-Dimensional Organ Culture Systems -- Organotypic Slice Explant Roller-Tube Cultures -- Neurons -- Astrocytes -- Oligodendrocytes -- Brain Capillaries.
520			_aMost cells will survive removal from the natural mic- environment of their in vivo tissue and placement into a sterile culture dish under optimal conditions. Not only do they survive, but they also multiply and express differen- ated properties in such a culture dish. A few cells do this in suspension, but most will need some kind of mechanical support substituting for their natural connections with other cells. The surface of a culture dish that might have to be coated is usually sufficient. The recent trend to standa- ization of conditions and the existence of commercial ent- prises with adequate funds and specializing in the needs of scientists were responsible for the tremendous proliferation of cell culture techniques in all fields of research in the last 20 years. No longer does a scientist have to concentrate all his/her efforts on that technology; the new trends make it feasible to employ cell culture techniques as only one of the many methods available in a small corner of a larger research laboratory. Some areas of research depend more heavily than others on cell culture techniques. Neuroscience is one of the areas that has developed hand in hand with the prol- eration of cell culture methodology. Molecular biological aspects, cell differentiation and development, neurophy- ological and neurochemical studies, as well as investigations into the nature of various diseases are now to a large extent dependent on the use of cell cultures.
988			_aSpringer_Protocols_1992
650		7	_2embne _97393 _aBiología molecular
700	1		_aBoulton, Alan A _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aBaker, Glen B _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aWalz, Wolfgang _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9780896032149
776	0	8	_iPrinted edition: _z9780896033481
776	0	8	_iPrinted edition: _z9781489940209
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896032140 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
998			_db