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008			130531s2013 xxu| s |||| 0|eng d
020			_a9781627034388
024	7		_a10.1007/978-1-62703-438-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-VaUE
050			_aQH506 _b2013 EB
245	1	0	_aTandem Repeats in Genes, Proteins, and Disease _bMethods and Protocols _cedited by Danny M. Hatters, Anthony J. Hannan.
250			_a1st edition 2013
264		1	_aTotowa, NJ _bHumana Press _c2013
300			_a1 recurso en línea (XI, 258 páginas) _b55 ilustraciones, 23 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1017
505	0		_aLongitudinal Imaging and Analysis of Neurons Expressing Polyglutamine-Expanded Proteins -- Atomic Force Microscopy Assays for Evaluating Polyglutamine Aggregation in Solution and on Surfaces -- Morphometric Analysis of Huntington's Disease Neurodegeneration in Drosophila -- Size Analysis of Polyglutamine Protein Aggregates using Fluorescence Detection in an Analytical Ultracentrifuge -- A Method for the Incremental Expansion of Polyglutamine Repeats in Recombinant Proteins -- Pulse Shape Analysis (PulSA) to Track Protein Translocalization in Cells by Flow Cytometry: Applications for Polyglutamine Aggregation -- Characterizing Social Behavior in Genetically Targeted Mouse Models of Brain Disorders -- PCR Amplification and Sequence Analysis of GC-rich Sequences: Aristaless-Related Homeobox Example -- Challenges of "sticky" Co-Immunoprecipitation; Polyalanine Tract Protein-Protein Interactions -- Molecular Pathology of Polyalanine Expansion Disorders-new Perspectives from Mouse Models -- Yeast as a Platform to Explore Polyglutamine Toxicity and Aggregation -- Immunobased Detection Assays to Quantify Distinct Mutant Huntingtin Conformations in Biological Samples -- Modeling and Analysis of Repeat RNA Toxicity in Drosophila -- Analyzing Modifiers of Protein Aggregation in C. elegans by Native Agarose Gel Electrophoresis -- Kinetic Analysis of Aggregation Data -- A Bioinformatics Method for Identifying Q/N-rich Prion-like Domains in Proteins -- Detecting Soluble PolyQ Oligomers and Investigating their Impact on Living Cells using Split-GFP -- Cell Biological Approaches To Investigate Polyglutamine-Expanded AR Metabolism.
520			_aThe genomes of humans, as well as many other species, are interspersed with hundreds of thousands of tandem repeats of DNA sequences.  Those tandem repeats located as codons within open reading frames encode amino acid runs, such as polyglutamine and polyalanine.  Tandem repeats have not only been implicated in biological evolution, development and function but also in a large collection of human disorders. In Tandem Repeats in Genes, Proteins, and Disease: Methods and Protocols, expert researchers in the field detail many methods covering the analysis of tandem repeats in DNA, RNA and protein, in healthy and diseased states.  This will include molecular genetics, molecular biology, biochemistry, proteomics, biophysics, cell biology, and molecular  and cellular approaches to animal models of tandem repeat disorders. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoratative and Practical, Tandem Repeats in Genes, Proteins, and Disease: Methods and Protocols aids scientists in continuing to study the unique methodological challenges that come from repetitive DNA and poly-amino acid sequences.
988			_aSpringer_Protocols_2013
650		7	_2embne _97393 _aBiología molecular
700	1		_aHatters, Danny M _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aHannan, Anthony J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781627034395
776	0	8	_iPrinted edition: _z9781627034371
776	0	8	_iPrinted edition: _z9781493963003
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-438-8 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
998			_db