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020			_a9781592599400
024	7		_a10.1385/1592599400 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-VaUE
050			_aRB150.F52 _b2008 EB
245	1	0	_aFibrosis Research _bMethods and Protocols _cedited by John Varga, David A. Brenner, Sem H. Phan.
264		1	_aTotowa, NJ _bHumana Press _c2008
300			_a1 recurso en línea (XVI, 392 páginas) _b80 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Medicine _x1940-6037 _v117
505	0		_aThe Clinical Scope of Fibrosis -- Pulmonary Fibrosis -- Renal Fibrosis -- Transforming Growth Factor-? -- Regulation of Extracellular Matrix Metabolism: Cultured Cells -- Isolation and Culture of Skin Fibroblasts -- Isolation and Culture of Hepatic Stellate Cells -- Isolation and Phenotypic Characterization of Lung Fibroblasts -- Methods for Measuring Type I Collagen Synthesis In Vitro -- Methods for Assessing the Molecular Mechanisms Controlling Gene Regulation -- Methods for Measuring TGF-? Using Antibodies, Cells, and Mice -- Studying Fibrosis Using Animal Models -- Morphological Methods for Assessment of Fibrosis -- Methods for Measuring Hydroxyproline and Estimating In Vivo Rates of Collagen Synthesis and Degradation -- Approaches to Evaluation of Fibrogenic Pathways in Surgical Lung Biopsy Specimens -- Animal Models for Adult Dermal Wound Healing -- Modeling Liver Fibrosis in Rodents -- Animal Models of Pulmonary Fibrosis -- Animal Models of Renal Fibrosis -- Animal Models of Cardiac Fibrosis -- Genetic Approaches to Studying Fibrosis -- Finding Fibrosis Genes -- Genetic Studies to Identify Hepatic Fibrosis Genes and SNPs in Human Populations -- Analysis of Microarray Experiments for Pulmonary Fibrosis -- DNA Microarrays and Data Mining To Study Hepatic Fibrosis -- Analysis of Proteins Dominantly Expressed in Hepatic Stellate Cells of Activated Phenotype.
520			_aFibrosis, or scar tissue, is increasingly recognized as an important feature of many chronic diseases of the lung, kidney, heart, skin, eye, and bone. With some 45% of deaths in the United States attributed to fibrosing disorders and the lack of any effective treatment, research into the cellular, molecular, and genetic basis of fibrosis has exploded. In Fibrosis Research: Methods and Protocols, leading investigators at the forefront of this emerging field review the highlights of current fibrosis work in a variety of disciplines and the experimental methodologies used to uncover the mechanisms that drive it. In their discussion of research techniques utilizing cultured cells to model various aspects of the fibrotic response in vitro, the authors describe the isolation, characterization, and propagation of mesenchymal cells, and highlight the similarities and differences between methods that are appropriate for different types of fibroblasts. Approaches for studying collagen gene regulation and TGF-b production are also discussed, along with experimental methodologies utilizing animal models to study the pathogenesis of fibrosis. Additional techniques presented cover the evolving genetic methods for identifying "fibrosis genes" or allelic polymorphisms in human populations, microarray studies for describing global patterns of gene expression associated with fibrosis, and proteomic approaches to the same. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Authoritative and state-of-the-art, Fibrosis Research: Methods and Protocols offers investigators all the powerful experimental methodologies, techniques, and strategies needed today to study and ultimately understand the daunting problem of pathological fibrosis.
988			_aSpringer_Protocols_2005
650		7	_2embne _9273311 _aMedicina
700	1		_aVarga, John _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aBrenner, David A _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aPhan, Sem H _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617375927
776	0	8	_iPrinted edition: _z9781588294791
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592599400 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
998			_db