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001			232973
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007			cr nn 008mamaa
008			100301s2009 xxu| s |||| 0|eng d
020			_a9781597455534
024	7		_a10.1007/978-1-59745-553-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aDNA and RNA Profiling in Human Blood _bMethods and Protocols _cedited by Peter Bugert.
250			_a1st edition 2009
264		1	_aTotowa, NJ _bHumana Press _c2009
300			_a1 recurso en línea (XI, 341 páginas) _b63 ilustraciones, 1 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v496
505	0		_aDNA Profiling for Blood Cell Antigens -- PCR-ELISA for High-Throughput Blood Group Genotyping -- Single Base Extension in Multiplex Blood Group Genotyping -- Real-Time PCR Assays for High-Throughput Blood Group Genotyping -- Real-Time PCR Assays for High-Throughput Human Platelet Antigen Typing -- Multiplex ABO Genotyping by Minisequencing -- Multiplex Genotyping for Thrombophilia-Associated SNPs by Universal Bead Arrays -- Pyrosequencing of Toll-Like Receptor Polymorphisms of Functional Relevance -- Multiplex HLA-Typing by Pyrosequencing -- High-Throughput Multiplex HLA-Typing by Ligase Detection Reaction (LDR) and Universal Array (UA) Approach -- Medium- to High-Throughput SNP Genotyping Using VeraCode Microbeads -- The Use of Maternal Plasma for Prenatal RhD Blood Group Genotyping -- RNA Profiling in Blood Cells -- Nanoliter High-Throughput PCR for DNA and RNA Profiling -- RNA Stabilization of Peripheral Blood and Profiling by Bead Chip Analysis -- RNA Profiling in Peripheral Blood Cells by Fluorescent Differential Display PCR -- cDNA Amplification by SMART-PCR and Suppression Subtractive Hybridization (SSH)-PCR -- Transcript Profiling of Human Platelets Using Microarray and Serial Analysis of Gene Expression (SAGE) -- Genome-Wide Platelet RNA Profiling in Clinical Samples -- PCR-Based Amplification of Platelet mRNA Sequences Obtained From Small-Scale Platelet Samples -- MicroRNA Profiling of Megakaryocytes -- Serial Analysis of Gene Expression Adapted for Downsized Extracts (SAGE/SADE) Analysis in Reticulocytes -- Real-Time PCR Analysis for Blood Cell Lineage Specific Markers -- Monitoring the Immune Response Using Real-Time PCR.
520			_aBlood samples have consistently proven to be a key source of genetic material for a wide variety of diagnostic or research purposes. In DNA and RNA Profiling in Human Blood: Methods and Protocols, leading international experts contribute both established and recently developed protocols for complex and high-throughput DNA and RNA profiling. Divided into two thorough sections, the volume concentrates on DNA profiling for blood cell antigens through methods on high-throughput multiplex approaches and SNP typing, along with RNA profiling in blood cells addressing certain blood cell types such as platelets, reticulocytes, and megakaryocytes. Written in the highly successful Methods in Molecular Biology™ series format, all of the chapters include brief introductions on the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as the Notes section which highlights tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA and RNA Profiling in Human Blood: Methods and Protocols is an ideal guide to the molecular profiling approaches that have opened up this broad field of research and have shown great promise in the further identifying of disease markers in blood.
700	1		_aBugert, Peter _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781603279864
776	0	8	_iPrinted edition: _z9781934115930
776	0	8	_iPrinted edition: _z9781627039130
776	0	8	_iPrinted edition: _z9781603275606
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-553-4 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2009
999			_c232973 _d232973