000			04256nam a22003735i 4500
001			232975
003			ES-VaUE
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007			cr nn 008mamaa
008			170503s2017 xxu| s |||| 0|eng d
020			_a9781493969609
024	7		_a10.1007/978-1-4939-6960-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aCell Viability Assays _bMethods and Protocols _cedited by Daniel F. Gilbert, Oliver Friedrich.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XII, 299 páginas) _b70 ilustraciones, 50 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1601
505	0		_aBasic Colorimetric Proliferation Assays: MTT, WST and Resazurin -- Assaying Cellular Viability using the Neutral Red Uptake Assay -- Assessment of Cell Viability with Single, Dual, and Multi-Staining Methods using Image Cytometry -- High-throughput Spheroid Screens using Volume, Resazurin Reduction, and Acid Phosphatase Activity -- A Protocol for in vitro High-throughput Chemical Susceptibility Screening in Differentiating NT2 Stem Cells -- Ferroptosis and Cell Death Analysis by Flow Cytometry -- Assaying Mitochondrial Respiration as an Indicator of Cellular Metabolism and Fitness -- An ATP-based Luciferase Viability Assay for Animal African Trypanosomes using a 96-well Plate -- SYBRâ Green I Based Fluorescence Assay to Assess Cell Viability of Malaria Parasites for Routine Use in Compound Screening -- Screening Applications to Test Cellular Fitness in Transwell® Models after Nanoparticle Treatment -- Assays for Analyzing the Role of Transport Proteins in the Uptake and the Vectorial Transport of Substances Affecting Cell Viability -- Metabolite Profiling Of Mammalian Cell Culture Processes to Evaluate Cellular Viability -- Assaying Spontaneous Network Activity and Cellular Viability using Multi-well Microelectrode Arrays -- Quantitative Ratiometric Ca2+ Imaging to Assess Cell Viability -- Functional Viability: Measurement of Synaptic Vesicle Pool Sizes -- Phenotyping Cellular Viability by Functional Analysis of Ion Channels: GlyR-targeted Screening in NT2-N Cells -- Systematic Cell-Based Phenotyping of Missense Alleles -- Second Harmonic Generation Microscopy of Muscle Cell Morphology and Dynamics -- Assessment of Population and ECM Production Using Multiphoton Microscopy as an Indicator of Cell Viability -- Average Rheological Quantities of Cells in Monolayers -- Measurement of Cellular Behavior by Electrochemical Impedance Sensing -- Nano-QSAR Model for Predicting Cell Viability of Human Embryonic Kidney Cells.
520			_aThis volume provides an overview of commonly used methods and protocols for cell fitness indicators. Chapters detail biochemical, fluorescence and luminescence-based strategies, computational, and label-free methodologies for assaying cellular viability by means of e.g. viscoelastic properties, impedance and multiphoton microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cell Viability Assays: Methods and Protocols aims to ensure successful results in the further study of this vital field.
700	1		_aGilbert, Daniel F _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aFriedrich, Oliver _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493969593
776	0	8	_iPrinted edition: _z9781493969616
776	0	8	_iPrinted edition: _z9781493983520
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6960-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c232975 _d232975