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008			110922s2012 xxu| s |||| 0|eng d
020			_a9781617792922
024	7		_a10.1007/978-1-61779-292-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aGene Regulatory Networks _bMethods and Protocols _cedited by Bart Deplancke, Nele Gheldof.
250			_a1st edition 2012
264		1	_aTotowa, NJ _bHumana Press _c2012
300			_a1 recurso en línea (XI, 446 páginas) _b108 ilustraciones, 33 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v786
505	0		_aHow Do You Find Transcription Factors? Computational Approaches to Compile and Annotate Repertoires of Regulators for Any Genome -- Expression Pattern Analysis of Regulatory Transcription Factors in Caenorhabditis elegans -- High-throughput SELEX Determination of DNA Sequences Bound by Transcription Factors In vitro -- Convenient Determination of Protein-Binding DNA Sequences using Quadruple 9-Mer-Based Microarray and DsRed-Monomer Fusion Protein -- Analysis of Specific Protein-DNA Interactions by Bacterial One-Hybrid Assay -- MITOMI- A Microdluidic Platform for In vitro Characterization of Transcription Factor - DNA Interaction -- Detecting Protein-protein Interactions with the Split-Ubiquitin Sensor -- Genome-wide Dissection of Post-transcriptional and Post-translational Interactions -- Linking Cellular Signalling to Gene Expression using EXT Encoded Reporter Libraries -- Sample Preparation for Small RNA Massive Parallel Sequencing -- CAGE-Cap Analysis Gene Expression: A Protocol for the Detection of Promoter and Transcriptional Networks -- Detecting DNasel-hypersensitivity Sites with MLPA -- Detecting Long-range Chromatin Interactions using the Chromosome Conformation Capture Sequencing (3C-seq) Method -- Analyzing Transcription Factor Occupancy during Embryo Development using ChIP-seq -- Genome-wide Profiling of DNA Binding Proteins using Barcode-based Multiplex Solexa Sequencing -- Computational Analysis of Protein-DNA Interactions from ChIP-seq Data -- Using a Yeast Inverse One-hybrid System to Identify Functional Binding Sites of Transcription Factors -- Using cisTargetX to Predict Transcriptional Targets and Networks in Drosophila -- Proteomic Methodologies to Study Transcription Factor Function -- A High-throughput Gateway-compatible Yeast One-hybrid Screen to Detect Protein-DNA Interactions -- BioTapestry: A Tool to Visualize the Dynamic Properties of Gene Regulatory Networks -- Implicit Methods for Qualitative Modeling of Gene Regulatory Networks.
520			_aGene regulatory networks play a vital role in organismal development and function by controlling gene expression. With the availability of complete genome sequences, several novel experimental and computational approaches have recently been developed which promise to significantly enhance our ability to comprehensively characterize these regulatory networks by enabling the identification of respectively their genomic or regulatory state components, or the interactions between these two in unprecedented detail. Divided into five convenient sections, Gene Regulatory Networks: Methods and Protocols details how each of these approaches contributes to a more thorough understanding of the composition and function of gene regulatory networks, while providing a comprehensive protocol on how to implement them in the laboratory. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and accessible, Gene Regulatory Networks: Methods and Protocols aims to provide novices and experienced researchers alike with a comprehensive and timely toolkit to study gene regulatory networks from the point of data generation to processing, visualization, and modeling.
700	1		_aDeplancke, Bart _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aGheldof, Nele _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617792915
776	0	8	_iPrinted edition: _z9781617792939
776	0	8	_iPrinted edition: _z9781493958382
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-292-2 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2012
999			_c232977 _d232977