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001			232980
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008			140502s2014 xxu| s |||| 0|eng d
020			_a9781493906857
024	7		_a10.1007/978-1-4939-0685-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aShotgun Proteomics _bMethods and Protocols _cedited by Daniel Martins-de-Souza.
250			_a1st edition 2014
264		1	_aNew York, NY _bSpringer International Publishing _c2014
300			_a1 recurso en línea (XIV, 492 páginas) _b81 ilustraciones, 48 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1156
505	0		_aSurvey of Shotgun Proteomics -- LC-MALDI-TOF/TOF for Shotgun Proteomics -- Fully Automatable Multidimensional Reversed-Phase Liquid Chromatography with Online Tandem Mass Spectrometry -- GeLC-MS/MS Analysis of Complex Protein Mixtures -- IPG Strip-Based Peptide Fractionation for Shotgun Proteomics -- SILAC Yeast: From Labeling to Comprehensive Proteome Quantification -- Analysis of Proteome Dynamics in Mice by Isotopic Labeling -- Stable Isotope Labeling in Mammals (SILAM) -- Analysis of Individual Protein Turnover in Live Animals on a Proteome-Wide Scale -- Determining Protein Subcellular Localization in Mammalian Cell Culture with Biochemical Fractionation and iTRAQ 8-Plex Quantification -- Brain Quantitative Proteomics Combining GeLC-MS and Isotope-Coded Protein Labeling (ICPL) -- Employing TMT Quantification in a Shotgun-MS Platform -- Employing TMT Quantification in Shotgun-MS Proteomic Analysis: A Focus on Skeletal Muscle -- Spectral Counting Label-Free Proteomics -- Quantification of Proteins by Label Free LC-MSE -- Bioinformatics for Proteomics: Opportunities at the Interface Between the Scientists, Their Experiments and the Community -- Identification of DNA Damage Checkpoint-Dependent Protein Interactions in Saccharomyces cerevisiae Using Quantitative Mass Spectrometry -- Application of Shotgun Proteomics for Discovery-Driven Protein-Protein Interaction -- Mapping Protein Complexes Using Covalently Linked Antibodies and Isobaric Mass Tags -- Biomarker Verification Using Selected Reaction Monitoring and Shotgun Proteomics -- Use of Universal Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)-Based Selected Reaction Monitoring (SRM) Approach for Verification of Breast Cancer-Related Protein Markers -- The Secretome Analysis by High Throughput Proteomics and Multiple Reaction Monitoring (MRM) -- Preparation of Heteroelement-Incorporated and Stable Isotope-Labeled Protein Standards for Quantitative Proteomics -- One-Source Peptide/Phosphopeptide Ratio Standards for Accurate and Site-Specific Determination of the Degree of Phosphorylation -- Quantitative Glycoproteomics for N-Glycoproteome Profiling -- A Practical Recipe to Survey Phosphoproteomes -- Quantitation of the Phosphoproteome Using the Library Assisted eXtracted Ion Chromatogram (LAXIC) Strategy -- Fast, Efficient and Quality-Controlled Phosphopeptide Enrichment from Minute Sample Amounts Using Titanium Dioxide -- Quantifying Small Molecule-Induced Changes in Cellular Protein Expression and Post-Translational Modifications Using Isobaric Mass Tags -- Analysis of Protein Structure by Crosslinking Combined with Mass Spectrometry -- Top Down Proteomics by Means of Orbitrap Mass Spectrometry.
520			_aShotgun Proteomics: Methods and Protocols serves as a vital collection of protocols through which thousands of proteins can be simultaneously identified, quantified, and characterized in a high throughput manner.  Beginning with the history of proteomics centered on the vital role of mass spectrometry in its development, this detailed volume continues with chapters on sample pre-fractionation, in vivo and in vitro stable isotope labeling, label-free proteomics, informatics, protein-protein interactions, targeted proteomics, and post-translational modifications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Practical and comprehensive, Shotgun Proteomics: Methods and Protocols is an ideal and up-to-date guide for researchers seeking to understand the proteome of any given species.
700	1		_aMartins-de-Souza, Daniel _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493906840
776	0	8	_iPrinted edition: _z9781493906864
776	0	8	_iPrinted edition: _z9781493946952
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-0685-7 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2014
999			_c232980 _d232980