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001			232983
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008			160531s2016 xxu| s |||| 0|eng d
020			_a9781493937240
024	7		_a10.1007/978-1-4939-3724-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	4	_aThe Nuclear Receptor Superfamily _bMethods and Protocols _cedited by Iain J. McEwan, PhD.
250			_a2nd edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (X, 250 páginas) _b44 ilustraciones, 32 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1443
505	0		_aThe Nuclear Receptor Superfamily- At Thirty -- Lipid Homeostasis and Ligands for Liver X Receptors: Identification and Characterization -- Use of Differential Scanning Fluorimetry to Identify Nuclear Receptor Ligands -- Drug-Discovery Pipeline for Novel Inhibitors of the Androgen Receptor -- Use of BRET to Study Protein-Protein Interactions in vitro and in vivo -- Studying Steroid Receptor Complexes in the Cellular Environment -- Post-translational Modifications of Steroid Receptors: Phosphorylation -- Mapping protein-DNA Interactions using ChIP-exo and Illumina-based Sequencing -- Methods to Identify Chromatin Bound Protein Complexes: From Genome-Wide to Locus Specific Approaches -- Measuring the Expression of microRNAs Regulated by Androgens -- Methods for Identifying and Quantifying Mrna Expression of Androgen Receptor Splicing Variants in Prostate Cancer -- Harvesting Human Prostate Tissue Material and Culturing Primary Prostate Epithelial Cells -- In vivo Imaging of Nuclear Receptor Transcriptional Activity -- Development and Characterisation of Cell-Specific Androgen Receptor Knockout Mice.
520			_aThis volume aims to describe a complementary range of molecular, cell biological, and in vivo protocols used to investigate the structure-function of nuclear receptors, together with experimental approaches that may lead to new drugs to selectively target nuclear receptor-associated diseases. The Nuclear Receptor Superfamily, Second Edition will benefit those starting out in the nuclear receptor research field as well as to more established researchers who wish to apply different methods to a particular receptor or research problem. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Nuclear Receptor Superfamily, Second Edition aims to ensure successful results in the further study of this vital field.
700	1		_aMcEwan, PhD, Iain J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493937226
776	0	8	_iPrinted edition: _z9781493937233
776	0	8	_iPrinted edition: _z9781493981205
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-3724-0 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c232983 _d232983