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001			232984
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007			cr nn 008mamaa
008			100301s2007 xxu| s |||| 0|eng d
020			_a9781597455169
024	7		_a10.1007/978-1-59745-516-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aHedgehog Signaling Protocols _cedited by Jamila I. Horabin.
250			_a1st edition 2007
264		1	_aTotowa, NJ _bHumana Press _c2007
300			_a1 recurso en línea (XVII, 256 páginas) _b45 ilustraciones, 7 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v397
505	0		_aPurifying the Hedgehog Protein and its Variants -- Application of Sonic Hedgehog to the Developing Chick Limb -- Manipulation of Hedgehog Signaling in Xenopus by Means of Embryo Microinjection and Application of Chemical Inhibitors -- Isolation of Rat Telencephalic Neural Explants to Assay Shh GABAergic Interneuron Differentiation-Inducing Activity -- Genetic Analysis of the Vertebrate Hedgehog-Signaling Pathway Using Muscle Cell Fate Specification in the Zebrafish Embryo -- Efficient Manipulation of Hedgehog/GLI Signaling Using Retroviral Expression Systems -- Cell Surface Marker and Cell Cycle Analysis, Hedgehog Signaling, and Flow Cytometry -- Detecting Tagged Hedgehog with Intracellular and Extracellular Immunocytochemistry for Functional Analysis -- Confocal Analysis of Hedgehog Morphogenetic Gradient Coupled with Fluorescent In Situ Hybridization of Hedgehog Target Genes -- RNAi in the Hedgehog Signaling Pathway: pFRiPE, a Vector for Temporally and Spatially Controlled RNAi in Drosophila -- Germline Clone Analysis for Maternally Acting Drosophila Hedgehog Components -- Clonal Analysis of Hedgehog Signaling in Drosophila Somatic Tissues -- GAL4/UAS Targeted Gene Expression for Studying Drosophila Hedgehog Signaling -- Biochemical Fractionation of Drosophila Cells -- Using Immunoprecipitation to Study Protein-Protein Interactions in the Hedgehog-Signaling Pathway -- Sequence Analyses to Study the Evolutionary History and Cis-Regulatory Elements of Hedgehog Genes.
520			_aIn both vertebrates and invertebrates, Hedgehog signaling regulates the development of the tissues and structures of the body. In addition, many cancers and diseases arise as a result of misregulation of the pathway, which makes it an incredibly important system to understand. This volume examines various methods used in the analysis of the Hedgehog signaling pathway, bringing together different lines of analyses into one accessible and comprehensive text. This volume provides a single source for investigators, presenting several different experimental approaches, which are all varied and broad in nature, as well as procedures for a variety of different model systems. Researchers will find this volume to be an invaluble tool as they work towards deeper understanding of the underlying molecular events that the Hedgehog signaling pathway regulates. .
700	1		_aHorabin, Jamila I _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617377211
776	0	8	_iPrinted edition: _z9781588296924
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-516-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2007
999			_c232984 _d232984